Malignant transformation of Syrian hamster embryo (SHE) cells in culture by malachite green: an agent of environmental importance.

Simple item page
dc.contributor.authorMahudawala, D Men_US
dc.contributor.authorRedkar, A Aen_US
dc.contributor.authorWagh, Aen_US
dc.contributor.authorGladstone, Ben_US
dc.contributor.authorRao, K Ven_US
dc.date.accessioned2009-05-28T13:08:02Z
dc.date.available2009-05-28T13:08:02Z
dc.date.issued1999-09-25en_US
dc.description.abstractMalachite green (MG), consisting of green crystals with a metallic lustre, is very soluble in water and is highly cytotoxic to mammalian cells in culture and also acts as a liver tumour promoter. In view of its industrial importance and possible exposure to human beings, MG poses a potential environmental health hazard. Accordingly, we have studied the effect of MG on the formation of free radicals using Electron Spin Resonance (ESR) analysis with 5,5-dimethyl-1-pyrroline N-oxide (DMPO) as a spin trapping agent. ESR analysis showed formation of reactive free radicals during exposure of MG to Syrian hamster embryo (SHE) cells. As per mechanism-based toxicology in cancer risk assessment, the chemicals that have the potential to be metabolized to active free radical species could be human cancer hazards. So, we have investigated the effect of MG on the formation of Type II and Type III morphologically transformed foci using SHE cell transformation assay. MG induced dose related transformed foci. Some of these transformed foci were taken out using selective trypsinisation and established immortal cell lines. One of these immortal cell lines was characterized extensively. This immortal cell line showed enhanced DNA synthesis in the form of BrdU incorporation, increased presence of proliferating cell nuclear antigen (PCNA), bcl-2 and p53 proteins by immunohistochemistry. When these immortal cells were injected subcutaneously into nude mice, they developed tumors which were transplantable and histopathologically sarcomas. The present studies indicate that MG could be a potential candidate for two year chemical carcinogenesis rodent bioassays.en_US
dc.description.affiliationCellular Carcinogenesis Laboratory, Tata Memorial Hospital, Tata Memorial Centre, Mumbai, India.en_US
dc.identifier.citationMahudawala DM, Redkar AA, Wagh A, Gladstone B, Rao KV. Malignant transformation of Syrian hamster embryo (SHE) cells in culture by malachite green: an agent of environmental importance. Indian Journal of Experimental Biology. 1999 Sep; 37(9): 904-18en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/59006
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Transformation, Neoplastic --drug effectsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCricetinaeen_US
dc.subject.meshHumansen_US
dc.subject.meshMesocricetusen_US
dc.subject.meshMiceen_US
dc.subject.meshRosaniline Dyes --toxicityen_US
dc.titleMalignant transformation of Syrian hamster embryo (SHE) cells in culture by malachite green: an agent of environmental importance.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Indian Journal of Experimental Biology