Simple and economical assay systems for evaluation of phosphinothricin resistant transgenics of sorghum, Sorghum bicolor. (L.) Moench., and pearl millet, Pennisetum glaucum (L.) R. Br.

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dc.contributor.authorHarshavardhan, Den_US
dc.contributor.authorSantha, Ben_US
dc.contributor.authorRani, T Sen_US
dc.contributor.authorUlaganathan, Ken_US
dc.contributor.authorMadhulety, T Yen_US
dc.contributor.authorLaxminarayana, Cen_US
dc.contributor.authorSeetharama, Nen_US
dc.date.accessioned2009-05-28T12:24:09Z
dc.date.available2009-05-28T12:24:09Z
dc.date.issued2003-02-17en_US
dc.description.abstractFive simple and rapid methods for evaluation of sorghum and pearl millet transgenics resistant to herbicide phosphinothricin (used as selectable marker) were studied. For rapid in vitro selection, three assays (establishment of sensitivity curves for embryogenic calli, determination of lethal doses for seed germination, and a rapid screening of cut young leaves based on the colour change of the medium) were established. For rapid screening of transgenic progeny, effects of in vivo Basta leaf spray and dip tests were studied at three different morphological stages. For all the above assays, LD50, and LD100 values were higher for pearl millet than sorghum. However, in both the crops, genotype effect was not significant. The assays standardized in the study were found to be effective for rapid, economical and mass-scale identification and characterization of transgenic plants of sorghum and pearl millet.en_US
dc.description.affiliationGlobal Theme 1, ICRISAT P.O., Patancheru 502 324, India.en_US
dc.identifier.citationHarshavardhan D, Santha B, Rani TS, Ulaganathan K, Madhulety TY, Laxminarayana C, Seetharama N. Simple and economical assay systems for evaluation of phosphinothricin resistant transgenics of sorghum, Sorghum bicolor. (L.) Moench., and pearl millet, Pennisetum glaucum (L.) R. Br. Indian Journal of Experimental Biology. 2003 Feb; 41(2): 141-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/58033
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAminobutyric Acids --pharmacologyen_US
dc.subject.meshBiological Assay --economicsen_US
dc.subject.meshDrug Resistanceen_US
dc.subject.meshGerminationen_US
dc.subject.meshGlutamate-Ammonia Ligase --antagonists & inhibitorsen_US
dc.subject.meshHerbicides --pharmacologyen_US
dc.subject.meshPennisetum --drug effectsen_US
dc.subject.meshPlant Leaves --metabolismen_US
dc.subject.meshPlants, Genetically Modifieden_US
dc.subject.meshSorghum --drug effectsen_US
dc.subject.meshTransgenes --drug effectsen_US
dc.titleSimple and economical assay systems for evaluation of phosphinothricin resistant transgenics of sorghum, Sorghum bicolor. (L.) Moench., and pearl millet, Pennisetum glaucum (L.) R. Br.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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