Purification and Characterization of Endoglucanase from Sorghum (S. bi-color) and Millet (Pennisetum typhoides and Digitaria exilis) Malts.
dc.contributor.author | Ukairo, D I | |
dc.contributor.author | Nzelibe, H C | |
dc.contributor.author | Iheme, C I | |
dc.contributor.author | Udenze, E C | |
dc.contributor.author | Ihedimbu, C P | |
dc.contributor.author | Ezirim, A U | |
dc.date.accessioned | 2016-12-15T08:02:28Z | |
dc.date.available | 2016-12-15T08:02:28Z | |
dc.date.issued | 2015 | |
dc.description.abstract | Endoglucanase (EC3.2.1.4) from sorghum (S. bi-color) and millet (Pennisetum typhoides & Digitaria exilis) malts were purified to homogeneity through the methods of ammonium sulphate precipitation and gel filtration. Molecular mass of 35 KDa and 41 KDa were determined by SDS-PAGE. The purified enzymes catalyzed the hydrolysis of carboxy-methylcellulose with optimum activity at pH of 4.8, 5.0, 6.0, and temperature of 60ºC, 60ºC and 70ºC for Digitaria exilis, S. bi-color and Pennisetum typhoides respectively. More than 90% activity was retained in S. bi-color and Pennisetum typhoides and 73% activity in Digitaria exilis after 1.0 hour pre-incubation at 60ºC. Km values of 0.11, 0.09, 0.20 mM and Vmax 17.53, 15.0 and 11.10 U/mg/min were obtained for S. bi-color, Pennisetum typhoides and Digitaria exilis respectively. Co2+ inhibited endoglucanase activity whereas Ca2+, Ba2+, and Zn2+ enhanced enzyme activity. The enzyme was inactivated by glucose, a major end product of cellulose hydrolysis. Results indicate that endoglucanase of S. bi-color and Pennisetum typhoides are more suitable for malting and a blend of the two will produce high quality malt. | en_US |
dc.identifier.citation | Ukairo D I, Nzelibe H C, Iheme C I, Udenze E C, Ihedimbu C P, Ezirim A U. Purification and Characterization of Endoglucanase from Sorghum (S. bi-color) and Millet (Pennisetum typhoides and Digitaria exilis) Malts. International Journal of Biochemistry Research & Review 2015 8(4): 1-11. | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/179891 | |
dc.language.iso | en | en_US |
dc.source.uri | https://www.sciencedomain.org/metrics/12052 | en_US |
dc.subject | Endoglucanase | en_US |
dc.subject | sorghum | en_US |
dc.subject | millet | en_US |
dc.subject | malting | en_US |
dc.subject | enzyme purification | en_US |
dc.title | Purification and Characterization of Endoglucanase from Sorghum (S. bi-color) and Millet (Pennisetum typhoides and Digitaria exilis) Malts. | en_US |
dc.type | Article | en_US |
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