Histo-architechtural Evaluation of Conventional Versus Two Rapid Microwave Processing Techniques.
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Date
2015
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Abstract
Aims: This work aims to reduce the time of tissue processing, exclude xylene from tissue
processing as well as to reduce the total quantity of reagents used per cycle of paraffin wax
processing technique.
Study Design: Harvesting and fixation of tissues. Grossing into triplicates, processing using three
different techniques. Staining and grading of sections.
Place and Duration of Study: Apparently healthy rabbits from the animal house, National
Veterinary research Institute, Vom, Jos, Nigeria, between August and December, 2014.
Methodology: Two apparently healthy rabbits were sacrificed and the Trachea, Lungs, Heart, Liver,
Kidney, Stomach, Skin, Brain, and the Spleen were harvested and fixed in 10% buffered formalin for three days. They were grossed into triplicates, labeled and processed using the conventional,
microwave without vacuum and microwave with vacuum respectively. They were sectioned and
stained simultaneously using the haematoxylin and eosin staining technique as well as the Gordon
and Sweet’s method. They were graded as excellent if they permitted microscopy, fair if not very
good but can permit microscopy and poor if they cannot permit microscopy at all.
Results: There is a drastic reduction in the duration of processing as well as the quantity of
reagents used in the microwave techniques as compared to the conventional method. Xylene was
completely eliminated in the microwave techniques. Tissue histo-architechture, special features as
well as silver impregnation were clearly demonstrated without significant differences. Dye uptake as
well as section thickness were comparable among the three techniques.
Conclusion: Same-day turn-around is possible in histology, with reduced reagent consumption and
elimination of xylene, without compromising section quality, dye uptake or ability to reduce silver to
its metallic form. This will result to quick diagnosis hence quick intervention at a cheaper rate to both
laboratories and clients. It is a good innovation in forensic and diagnostic histopathology and should
be encouraged. Its compatibility with histochemical, immunohistochemical and molecular techniques
should be evaluated to give a wider application.
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Keywords
Tissue processing, xylem, isopropanol conventional method, microwave, vacuum, haematoxylin and eosin, silver impregnation
Citation
Choji Tobias Peter Pwajok, Ngokere Anthony Ajuluchukwu, Ogenyi Samuel lfedioranma, Kumbish Peterside Rinle. Histo-architechtural Evaluation of Conventional Versus Two Rapid Microwave Processing Techniques. British Biotechnology Journal. 2015 8(3): 1-19.