Sensitive and stable ethanol biosensor development based on Acetobacter aceti biofilm for halal detection of food and beverages

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dc.contributor.authorNinik, Triayu Susparinien_US
dc.contributor.authorZaenal, Abidinen_US
dc.contributor.authorDyah, Iswantinien_US
dc.contributor.authorNurhidayat, Noviken_US
dc.date.accessioned2020-10-16T08:50:16Z
dc.date.available2020-10-16T08:50:16Z
dc.date.issued2019-11
dc.description.abstractA highly sensitive ethanol biosensor based on Acetobacter aceti biofilm, which contains pyrroloquinolinequinone-alcohol dehydrogenase enzyme for halal detection in food and beverages, has been developed. In thisstudy, A. aceti cells were purified by determining the conditions of growth time, incubation temperature, andpH to suppress the yeast growth. Selection of optimum conditions for biofilm formation was also carried out toobtain a stable sensor operation. The optimum biofilm formation was 14 days. The performance of biosensor wasinvestigated by the cyclic voltammetry technique. Linear range, sensitivity, limit of detection (LOD), and limitof quantification (LOQ) were found to be 5 × 10−5–3 × 10−4 %, 43,076 µA %−1, 2.32 × 10−5, and 7.03 × 10−5 %,respectively. The repeatability of biosensor was tested in 1 × 10−5 − 1% ethanol concentration and the relativestandard deviation was 1.08% (for n = 7). The stability studies have shown that the cyclic voltammetry responseof biosensor to ethanol leave the act of 98.99% at the 7 week. Thus, the developed biosensor is promising to beused for simple analysis of halal products and has been tested on real sample.en_US
dc.identifier.affiliationsDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Jalan Agatis Kampus IPB Dramaga, Bogor, Indonesiaen_US
dc.identifier.affiliationsDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Jalan Agatis Kampus IPB Dramaga, Bogor, Indonesiaen_US
dc.identifier.affiliationsBiopharmaca Research Center, Bogor Agricultural University, Jalan Taman Kencana No. 3, Bogor, Indonesiaen_US
dc.identifier.affiliationsDivision of Microbiology Research Center for Biology, The Indonesian Institute of Sciences, Jalan Raya Jakarta-Bogor Km 46 Cibinong, Bogor, Indonesiaen_US
dc.identifier.citationNinik Triayu Susparini, Zaenal Abidin, Dyah Iswantini, Nurhidayat Novik. Sensitive and stable ethanol biosensor development based on Acetobacter aceti biofilm for halal detection of food and beverages. Journal of Applied Biology & Biotechnology. 2025 Nov; 2025 Nov: 40-47en_US
dc.identifier.issn2455-7005
dc.identifier.issn2347-212X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/209858
dc.languageenen_US
dc.publisherOpen Science Publishers LLPen_US
dc.relation.issuenumber6en_US
dc.relation.volume7en_US
dc.source.urihttps://dx.doi.org//10.7324/JABB.2019.70607en_US
dc.subjectEthanolen_US
dc.subjectBiosensoren_US
dc.subjectA. acetien_US
dc.subjectBiofilmen_US
dc.subjectPQQ-ADHen_US
dc.subjectHalalen_US
dc.titleSensitive and stable ethanol biosensor development based on Acetobacter aceti biofilm for halal detection of food and beveragesen_US
dc.typeJournal Articleen_US
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