Purification of elastase-like chymotrypsin from cardamom shoot and Capsule borer [corrected]

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dc.contributor.authorJosephrajkumar, Aen_US
dc.contributor.authorChakrabarty, Ren_US
dc.contributor.authorThomas, Gen_US
dc.date.accessioned2009-05-28T10:51:40Z
dc.date.available2009-05-28T10:51:40Z
dc.date.issued2007-11-13en_US
dc.description.abstractAn elastase-like chymotrypsin was purified by aprotinin-agarose affinity chromatography from the midgut extract of cardamom shoot and capsule borer, Conogethes punctiferalis. The purified enzyme had a Vmax of 687.6 +/- 22.1 nmole pNA released/min/mg protein, Km of 0.168 +/- 0.012 mM with SAAPLpNA as substrate and gave a single band on SDS-PAGE with a molecular mass of 72.1 kDa. Casein zymogram revealed one clear zone of proteolytic activity, which corresponded to the band obtained with SDS-PAGE indicating that this could be a single-polypeptide enzyme.en_US
dc.description.affiliationCardamom Research Station, Kerala Agricultural University, Pampadumpara 685 556, Dist. Idukki, India. entojoe2003@yahoo.co.inen_US
dc.identifier.citationJosephrajkumar A, Chakrabarty R, Thomas G. Purification of elastase-like chymotrypsin from cardamom shoot and Capsule borer [corrected] Indian Journal of Experimental Biology. 2007 Nov; 45(11): 998-1002en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/55901
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/5359/1/IJEB%2045%2811%29%20998-1002.pdfen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAprotinin --pharmacologyen_US
dc.subject.meshChromatography, Agaroseen_US
dc.subject.meshChymotrypsin --isolation & purificationen_US
dc.subject.meshDigestive System --enzymologyen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshElettaria --parasitologyen_US
dc.subject.meshFruit --parasitologyen_US
dc.subject.meshLarvaen_US
dc.subject.meshLepidoptera --enzymologyen_US
dc.subject.meshPancreatic Elastase --isolation & purificationen_US
dc.subject.meshPlant Shoots --parasitologyen_US
dc.subject.meshProtein Conformationen_US
dc.subject.meshSerine Proteinase Inhibitors --pharmacologyen_US
dc.titlePurification of elastase-like chymotrypsin from cardamom shoot and Capsule borer [corrected]en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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