In vitro validation of self designed “universal human Influenza A siRNA”.

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dc.contributor.authorJain, Bhawana
dc.contributor.authorJain, Amita
dc.contributor.authorPrakash, Om
dc.contributor.authorSingh, Ajay Kr
dc.contributor.authorDangi, Tanushree
dc.contributor.authorSingh, Mastan
dc.contributor.authorSingh, KP
dc.date.accessioned2016-11-18T07:33:08Z
dc.date.available2016-11-18T07:33:08Z
dc.date.issued2015-08
dc.description.abstractThe genomic variability of Influenza A virus (IAV) makes it difficult for the existing vaccines or anti-influenza drugs to control. The siRNA targeting viral gene induces RNAi mechanism in the host and silent the gene by cleaving mRNA. In this study, we developed an universal siRNA and validated its efficiency in vitro. The siRNA was designed rationally, targeting the most conserved region (delineated with the help of multiple sequence alignment) of M gene of IAV strains. Three level screening method was adopted, and the most efficient one was selected on the basis of its unique position in the conserved region. The siRNA efficacy was confirmed in vitro with the Madin Darby Canine Kidney (MDCK) cell line for IAV propagation using two clinical isolates i.e., Influenza A/H3N2 and Influenza A/pdmH1N1. Of the total 168 strains worldwide and 33 strains from India, 97 bp long (position 137-233) conserved region was identified. The longest ORF of matrix gene was targeted by the selected siRNA, which showed 73.6% inhibition in replication of Influenza A/pdmH1N1 and 62.1% inhibition in replication of Influenza A/H3N2 at 48 h post infection on MDCK cell line. This study provides a basis for the development of siRNA which can be used as universal anti-IAV therapeutic agent.en_US
dc.identifier.citationJain Bhawana, Jain Amita, Prakash Om, Singh Ajay Kr, Dangi Tanushree, Singh Mastan, Singh KP. In vitro validation of self designed “universal human Influenza A siRNA”. Indian Journal of Experimental Biology. 2015 Aug; 53(8): 514-521.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/178548
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/31910en_US
dc.subjectAnti-IAV siRNAen_US
dc.subjectH1N1en_US
dc.subjectH3N2en_US
dc.subjectIAVen_US
dc.subjectMultiple sequence alignmenten_US
dc.subjectMDCK cell lineen_US
dc.subjectsiRNA efficacyen_US
dc.titleIn vitro validation of self designed “universal human Influenza A siRNA”.en_US
dc.typeArticleen_US
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