Ca2+ -free medium enhances the magnitude of slow peak in compound action potential of frog sciatic nerve in vitro.

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dc.contributor.authorMandal, Maloy Ben_US
dc.contributor.authorDeshpande, Shripad Ben_US
dc.date.accessioned2009-05-28T16:07:30Z
dc.date.available2009-05-28T16:07:30Z
dc.date.issued2004-01-28en_US
dc.description.abstractThe present investigation was carried out to know the effect of Ca2+ on different peaks of compound action potential (CAP) representing the fibers having different conduction velocity. CAP was recorded from a thin bundle of nerve fibers obtained from desheathed frog sciatic nerve. Suction electrodes were used for stimulating and recording purposes. In Ca2+ -free amphibian Ringer, two distinct peaks (Peak-I and Peak-II) were observed. The threshold, conduction velocity (CV), amplitude and duration of Peak-I were 0.32 +/- 0.02 V, 56 +/- 3.0 m/sec, 2.1 +/- 0.2 mV and 0.75 +/- 0.1 ms, respectively. The Peak-II exhibited ten times greater threshold, eight times slower CV, three times lower amplitude and four times greater duration as compared to Peak-I. Addition of 2 mM Ca2+ in the bathing medium did not alter CAP parameters of Peak-I excepting 25% reduction in CV. But, in Peak-II there was 70-75% reduction in area and amplitude. The concentration-attenuation relation of Peak-II to various concentrations of Ca2+ was nonlinear and 50% depression occurred at 0.35 mM of Ca2+. Washing with Ca2+ -free solution with or without Mg2+ (2 mM)/verapamil (10 microM) could not reverse the Ca2+ -induced changes in Peak-II. Washing with Ca2+ -free solution containing EDTA restored 70% of the response. The results indicate that Ca2+ differentially influence fast and slow conducting fibers as the activity of slow conducting fibers is greatly suppressed by external calcium.en_US
dc.description.affiliationDepartment of Physiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India. maloy_mandal@yahoo.comen_US
dc.identifier.citationMandal MB, Deshpande SB. Ca2+ -free medium enhances the magnitude of slow peak in compound action potential of frog sciatic nerve in vitro. Indian Journal of Experimental Biology. 2004 Jan; 42(1): 43-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/62838
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAction Potentialsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCalcium --antagonists & inhibitorsen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshEdetic Acid --pharmacologyen_US
dc.subject.meshElectrodesen_US
dc.subject.meshElectrophysiology --methodsen_US
dc.subject.meshMagnesium --chemistryen_US
dc.subject.meshRanidaeen_US
dc.subject.meshSciatic Nerve --metabolismen_US
dc.subject.meshTime Factorsen_US
dc.titleCa2+ -free medium enhances the magnitude of slow peak in compound action potential of frog sciatic nerve in vitro.en_US
dc.typeJournal Articleen_US
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