An ELISA method for quantitation of tubulin using poly-1-lysine coated microtiter plates.

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dc.contributor.authorDas, Sen_US
dc.contributor.authorBanerjee, S Ken_US
dc.contributor.authorSil, Men_US
dc.contributor.authorSarkar, P Ken_US
dc.date.accessioned2009-05-28T11:28:52Z
dc.date.available2009-05-28T11:28:52Z
dc.date.issued1989-11-01en_US
dc.description.abstractA new, sensitive and convenient ELISA method has been developed for quantitation of tubulin using poly-1-lysine (PLL) coated multiwell microtiter plates. Binding of tubulin to untreated plastic surface of microtiter plates was extremely poor. Coating of wells with PLL enhanced the binding and facilitated quantitation by ELISA. Binding of tubulin was followed by stepwise additions of rabbit anti-tubulin IgG, HRP-conjugated goat anti-rabbit IgG and colour reagent. The method has been successfully applied to quantitate the tubulin content of extracts from rat brain and liver as evident from the excellent correlation of the results with those obtained from 3H-colchicine binding assay. The detection limit is as low as 5 ng, which is relatively better than that of the previous RIA methods. The ELISA method does not involve the use of any radioactive compound and all reagents required for this assay are commercially available.en_US
dc.identifier.citationDas S, Banerjee SK, Sil M, Sarkar PK. An ELISA method for quantitation of tubulin using poly-1-lysine coated microtiter plates. Indian Journal of Experimental Biology. 1989 Nov; 27(11): 972-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/56773
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBinding, Competitiveen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay --instrumentationen_US
dc.subject.meshPolylysineen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshRatsen_US
dc.subject.meshTubulin --analysisen_US
dc.titleAn ELISA method for quantitation of tubulin using poly-1-lysine coated microtiter plates.en_US
dc.typeJournal Articleen_US
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