Broad spectrum antibacterial activity of granaticinic acid, isolated from Streptomyces thermoviolaceus NT1; an endophyte in Catharanthus roseus (L.) G. Don.
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Date
2015-07
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Abstract
Searching for endophytic actinomycetes, strain NT1 was isolated from surface sterilized stem of Catharanthus
roseus (L.) G. Don collected from Paschim Medinipur, India. The strain was identified as Streptomyces
thermoviolaceus NT1 on the basis of morphological, biochemical and 16s rDNA based phylogenetic analysis. It
showed potential antagonism against several Gram positive and Gram negative bacterial pathogens along with
drug resistant Staphylococcus aureus. Maximum antibacterial production was obtained in ISP2 media at pH 7.2,
35 °C for 10 days. The active antibacterial substance was purified by Silica gel column chromatography and
activity guided TLC. IR and NMR analysis identified the active compound as granaticinic acid of m/z 463.26
[M+H]. These results suggest that the antimicrobial produced by the isolated endophytic strain will be useful in
near future.
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Keywords
Endophyte, Streptomyces thermoviolaceus, granaticinic acid, antibacterial, drug resistance
Citation
Roy Sudipta, Banerjee Debdulal. Broad spectrum antibacterial activity of granaticinic acid, isolated from Streptomyces thermoviolaceus NT1; an endophyte in Catharanthus roseus (L.) G. Don. Journal of Applied Pharmaceutical Science. 2015 July; 5(7): 6-11.