Detection, prevalence, purification and characterization of lecithinase of Klebsiella pneumoniae.

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dc.contributor.authorSingh, B Ren_US
dc.contributor.authorSharma, V Den_US
dc.contributor.authorChandra, Ren_US
dc.date.accessioned2009-05-28T12:00:17Z
dc.date.available2009-05-28T12:00:17Z
dc.date.issued1999-09-25en_US
dc.description.abstractLecithinase activity in Klebsiella is a rare trait as out of 208 strains of Klebsiella belonging to 3 species, viz. K. pneumoniae (168), K. planticola (29) and K. oxytoca (11), only 4 strains of K. pneumoniae produced lecithinase positive colonies on egg-yolk-agar. Although cell lysates of 16 K. pneumoniae yielded positive results for lecithinase assay on egg-yolk-agar, 19 strains were detected positive for lecithinase with ELISA using anti-lecithinase serum. Release of up to 52.12% cell-bound lecithinase could be achieved with polymyxin-B treatment at 100 micrograms/ml concentration. Purified lecithinase was determined to be a high molecular weight (70 kDa), crystalizable, anionic (pI, 3.5) protein. It possessed cytolytic, haemolytic and dermonecrotic activities but did not induce fluid accumulation in rabbit ileal loop or infant mouse guts. It was inactivated by boiling, trypsin and chymotrypsin treatment and alkaline pH. Serologically, it was related to lecithinase of Aeromonas caviae and phospholipase-C of Salmonella.en_US
dc.description.affiliationDepartment of Veterinary Microbiology and Immunology, College of Veterinary Sciences, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, India.en_US
dc.identifier.citationSingh BR, Sharma VD, Chandra R. Detection, prevalence, purification and characterization of lecithinase of Klebsiella pneumoniae. Indian Journal of Experimental Biology. 1999 Sep; 37(9): 925-32en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/57493
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshKlebsiella pneumoniae --enzymologyen_US
dc.subject.meshMiceen_US
dc.subject.meshPhospholipases --isolation & purificationen_US
dc.subject.meshRabbitsen_US
dc.titleDetection, prevalence, purification and characterization of lecithinase of Klebsiella pneumoniae.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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