Kinetic properties of gamma-glutamyltranspeptidase and leucine-amino-peptidase in developing rat intestine: effect of hormones.

Simple item page
dc.contributor.authorKaur, Jen_US
dc.contributor.authorJaswal, V Men_US
dc.contributor.authorMahmood, Aen_US
dc.date.accessioned2009-05-28T11:11:47Z
dc.date.available2009-05-28T11:11:47Z
dc.date.issued1990-01-01en_US
dc.description.abstractAdministration of cortisone and thyroxine produced adult-type increase in the activities of soluble and membrane-bound gamma-glutamyltranspeptidase (gamma-GTP) in suckling rat intestine. Membrane-bound enzyme activity remained unaltered while the soluble enzyme activity was reduced (27%) in insulin-injected pups. Kinetic analysis revealed that the observed changes in the enzyme levels were a consequence of altered Vmax with no change in apparent Km. A 2-fold increase in the Km value was observed in adult gamma-GTP activity compared to that of suckling animals. Membrane-bound and soluble gamma-GTP yielded similar values of the Ea (9.7-13.1 kcal/mole) but exhibited apparent differences in heat stability in the control and hormone-injected groups. Leucine-amino peptidase(LAP) activity was reduced to adult levels in insulin-treated suckling animals. Thyroxine- and cortisone-treatment did not affect soluble activity but significantly (P less than 0.001) augmented the membrane-bound LAP levels. This increase was due to enhanced (54-82%) Vmax with no change in Km. The observed decrease in LAP activity in response to insulin was due to reduced Vmax. There was no change in Ea (8-11.6 kcal/mole) except the value was raised to 19.1 kcal/mole in cortisone-injected pups. Both the soluble and membrane-bound LAP activities were quite resistant to heat inactivation upto 30 min at 60 degrees C except in weanling rats. Thus, the kinetic behaviour of normally developed and precociously induced gamma-GTP and LAP is essentially similar but there are apparent differences in the mode of action of insulin, cortisone and thyroxine in affecting the development of these enzymes.en_US
dc.description.affiliationDepartment of Biochemistry, Panjab University, Chandigarh, India.en_US
dc.identifier.citationKaur J, Jaswal VM, Mahmood A. Kinetic properties of gamma-glutamyltranspeptidase and leucine-amino-peptidase in developing rat intestine: effect of hormones. Indian Journal of Experimental Biology. 1990 Jan; 28(1): 18-22en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/56374
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Sucklingen_US
dc.subject.meshCortisone --pharmacologyen_US
dc.subject.meshHormones --pharmacologyen_US
dc.subject.meshIntestines --enzymologyen_US
dc.subject.meshKineticsen_US
dc.subject.meshLeucyl Aminopeptidase --metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.subject.meshThyroxine --pharmacologyen_US
dc.subject.meshgamma-Glutamyltransferase --metabolismen_US
dc.titleKinetic properties of gamma-glutamyltranspeptidase and leucine-amino-peptidase in developing rat intestine: effect of hormones.en_US
dc.typeJournal Articleen_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Indian Journal of Experimental Biology