Differential gene expression analysis in germinating and dormant teliospores of Tilletia indica using RNA seq approach

Simple item page
dc.contributor.authorM, Haritha Mohanen_US
dc.contributor.authorGurjar, Malkhan Singhen_US
dc.contributor.authorJain, Prachien_US
dc.contributor.authorKumar, Nareshen_US
dc.contributor.authorSaharan, Mahender Singhen_US
dc.contributor.authorAggarwal, Rashmien_US
dc.date.accessioned2023-08-09T06:21:17Z
dc.date.available2023-08-09T06:21:17Z
dc.date.issued2023-03
dc.description.abstractKarnal bunt of wheat is an important quarantine disease that interrupts India’s wheat trade in the international market. The whole transcriptome of germinating and dormant teliospores of Tilletia indica was performed using the RNA Seq approach to identify germination-related genes. Approximately 63 million reads were generated using the RNA sequencing by the Illumina NextSeq500 platform. The high-quality reads were deposited in NCBI SRA database (accession: PRJNA522347). The unigenes from the pooled teliospores were 16,575 having unigenes length of 28,998,753 bases. The high-quality reads of germinating teliospores mapped on to 21,505 predicted CDSs. 9,680 CDSs were common between dormant and germinating teliospores of T. indica. 11,825 CDSs were found to be in germinating teliospores while only 91 were unique in dormant spores of pathogen. The pathway analysis showed the highest number of pathways was found in germinating spores than dormant spores. The highest numbers of CDSs were found to be associated with translation (431 in number), transport and catabolism (340), signal transduction (326), and carbohydrate metabolism (283). The differential expression analysis (DESeq) of germinating and dormant teliospores showed that 686 CDS were up-regulated and 114 CDS were down-regulated in the germinating teliospores. Significant germination-related genes in the spores were validated using qPCR analysis. Ten genes viz. Ti3931, Ti6828, Ti7098, Ti7462, Ti7522, Ti 9289, Ti 8670, Ti 7959, Ti 7809,and Ti10095 were highly up-regulated in germinated teliospores which may have role in germination of spores.Further, these differentially expressed genes provide insights into the molecular events. This first study of transcriptome will be helpful to devise better management strategies to manage Karnal bunt disease.en_US
dc.identifier.affiliationsFungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi-110012 Indiaen_US
dc.identifier.affiliationsDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi-110012, Indiaen_US
dc.identifier.citationM Haritha Mohan, Gurjar Malkhan Singh, Jain Prachi, Kumar Naresh, Saharan Mahender Singh, Aggarwal Rashmi. Differential gene expression analysis in germinating and dormant teliospores of Tilletia indica using RNA seq approach. Indian Journal of Experimental Biology. 2023 Mar; 61(3): 214-223en_US
dc.identifier.issn0975-1009
dc.identifier.issn0019-5189
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/222589
dc.languageenen_US
dc.publisherCSIR-NIScPRen_US
dc.relation.issuenumber3en_US
dc.relation.volume61en_US
dc.source.urihttps://doi.org/10.56042/ijeb.v61i03.58626en_US
dc.subjectCoding sequenceen_US
dc.subjectGene ontologyen_US
dc.subjectKarnal bunten_US
dc.subjectTranscriptomeen_US
dc.titleDifferential gene expression analysis in germinating and dormant teliospores of Tilletia indica using RNA seq approachen_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
IJEB2023v61n3p214.pdf
Size:
6.11 MB
Format:
Adobe Portable Document Format
Download
Collections
Indian Journal of Experimental Biology