Comparative functional characterization of mouse bone marrow-derived mast cells and peritoneal mast cells in response to non-immunological stimuli.

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dc.contributor.authorSingh, Ren_US
dc.contributor.authorKumar, Pen_US
dc.contributor.authorGupta, P Pen_US
dc.date.accessioned2009-05-28T11:03:20Z
dc.date.available2009-05-28T11:03:20Z
dc.date.issued2001-04-09en_US
dc.description.abstractThe cultured mouse mast cells that are dependent on spleen-derived factor for their proliferation and maintenance and have been shown to be similar to mucosal mast cells in terms of their T-cell dependence and histochemical staining characteristics. Mast cell heterogeneity has been confirmed by functional characterization of mouse bone marrow-derived mast cells (MBMMC) and mouse peritoneal mast cells (MPMCs). MPMCs released around 30% of histamine when stimulated with compound 48/80 whereas MBMMC were almost unresponsive to the same stimulus. Calcium Ionophore A23187 on the other hand, released histamine in dose-dependent manner from MBMMC. The study was undertaken to investigate the effect of antiallergic drug, disodium cromoglycate (DSCG), a synthetic cromone and quercetin, a plant-derived flavonoid on Ca ionophore A23187 induced histamine release from MBMMC. MBMMCs were almost unresponsive to DSCG whereas Ca Ionophore induced histamine release was blocked by Quercetin. The results indicate that response of mast cells at one anatomic site to a given stimulus does not necessarily predict the response of mast cells at a different anatomic location to the same stimulus. It shows functional heterogeneity within a single species. So, it cannot be assumed that antiallergic compounds stabilizing mast cells in one tissue site or organ will be equally efficacious against mast cells in other sites.en_US
dc.description.affiliationDivision of Pharmacology, Central Drug Research Institute, Lucknow, India.en_US
dc.identifier.citationSingh R, Kumar P, Gupta PP. Comparative functional characterization of mouse bone marrow-derived mast cells and peritoneal mast cells in response to non-immunological stimuli. Indian Journal of Experimental Biology. 2001 Apr; 39(4): 323-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/56176
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBone Marrow Cells --drug effectsen_US
dc.subject.meshCalcimycin --pharmacologyen_US
dc.subject.meshCromolyn Sodium --pharmacologyen_US
dc.subject.meshHistamine Release --drug effectsen_US
dc.subject.meshMaleen_US
dc.subject.meshMast Cells --drug effectsen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred BALB Cen_US
dc.subject.meshPeritoneal Cavity --cytologyen_US
dc.subject.meshQuercetin --pharmacologyen_US
dc.subject.meshp-Methoxy-N-methylphenethylamine --pharmacologyen_US
dc.titleComparative functional characterization of mouse bone marrow-derived mast cells and peritoneal mast cells in response to non-immunological stimuli.en_US
dc.typeComparative Studyen_US
dc.typeIn Vitroen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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