Analytical Method Development For Simultaneous Determination Of Ubidecarenone And Vitamin E Acetate In Capsule Dosage Form By Hplc

Simple item page
dc.contributor.authorGrace, A. C.en_US
dc.contributor.authorPrabha, T.en_US
dc.contributor.authorJagadeeswaran, M.en_US
dc.contributor.authorSrinivasan, K.en_US
dc.contributor.authorSivakumar, T.en_US
dc.date.accessioned2020-09-24T08:00:47Z
dc.date.available2020-09-24T08:00:47Z
dc.date.issued2019-01
dc.description.abstractObjective: To develop and validate a new simple, accurate, precise and sensitive high performance liquid chromatographic method (HPLC) method for simultaneous estimation of ubidecarenone and vitamin E acetate in capsule dosage form as per international conference on harmonization (ICH) guidelines. Methods: The chromatographic separation of drugs were achieved using hypersil C8 column (250 mm x 4.6 mm, 5µ) in isocratic elution mode with a mobile phase of methanol: ethanol: n-hexane (80:10:10 v/v/v) at a flow rate of 1 ml/min with ultra-violet (UV) detection at 210 nm. Results: The optimized method produced sharp peaks with good resolution, minimum tailing factor and satisfactory retention time were found to be 5.745 min and 12.565 min for vitamin E acetate and ubidecarenone respectively. The method was linear in the range of 60-180 µg/ml for ubidecarenone and 20-60 µg/ml for vitamin E acetate with a correlation coefficient of 0.999 and 0.9993 respectively. Mean recoveries observed for ubidecarenone and vitamin E acetate were 99.85% and 99.73% respectively. The percentage relative standard deviation (% RSD) of peak area for system precision, method precision, and intermediate precision were found to be less than 0.37%. The lower degree of % RSD obtained has proved that the method was precise and robust. Conclusion: A new simple HPLC method was developed and validated as per ICH guidelines for the simultaneous estimation of ubidecarenone and vitamin E acetate and the method can be effectively applied for the routine analysis of active pharmaceutical ingredient (API) and formulations.en_US
dc.identifier.affiliationsDepartment of Pharmaceutical Analysis, Nandha college of Pharmacy, Erode-638 052, Tamilnadu, Indiaen_US
dc.identifier.affiliationsDepartment of Pharmaceutical Analysis, Nandha college of Pharmacy, Erode-638 052, Tamilnadu, Indiaen_US
dc.identifier.affiliationsDepartment of Pharmaceutical Analysis, Nandha college of Pharmacy, Erode-638 052, Tamilnadu, Indiaen_US
dc.identifier.affiliationsDepartment of Pharmaceutical Chemistry, Nandha college of Pharmacy, Erode-638 052, Tamilnadu, Indiaen_US
dc.identifier.affiliationsDepartment of Pharmaceutical Chemistry, Nandha college of Pharmacy, Erode-638 052, Tamilnadu, Indiaen_US
dc.identifier.citationGrace A. C., Prabha T., Jagadeeswaran M., Srinivasan K., Sivakumar T.. Analytical Method Development For Simultaneous Determination Of Ubidecarenone And Vitamin E Acetate In Capsule Dosage Form By Hplc. International Journal of Pharmacy and Pharmaceutical Sciences. 2019 Jan; 11(1): 79-84en_US
dc.identifier.issn0975-1491
dc.identifier.issn2656-0097
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/205809
dc.languageenen_US
dc.publisherInnovare Academic Sciences Pvt. Ltd.en_US
dc.relation.issuenumber1en_US
dc.relation.volume11en_US
dc.source.urihttps://doi.org/10.22159/ijpps.2019v11i1.30054en_US
dc.subjectUbidecarenoneen_US
dc.subjectVitamin E acetateen_US
dc.subjectHPLCen_US
dc.subjectMethod developmenten_US
dc.subjectCoenzyme-Q10en_US
dc.subjectalpha-tocopheryl acetateen_US
dc.titleAnalytical Method Development For Simultaneous Determination Of Ubidecarenone And Vitamin E Acetate In Capsule Dosage Form By Hplcen_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijpps2019v11n1p79.pdf
Size:
431.41 KB
Format:
Adobe Portable Document Format
Download
Collections
International Journal of Pharmacy and Pharmaceutical Sciences