Analytical Method Development For Simultaneous Determination Of Ubidecarenone And Vitamin E Acetate In Capsule Dosage Form By Hplc
dc.contributor.author | Grace, A. C. | en_US |
dc.contributor.author | Prabha, T. | en_US |
dc.contributor.author | Jagadeeswaran, M. | en_US |
dc.contributor.author | Srinivasan, K. | en_US |
dc.contributor.author | Sivakumar, T. | en_US |
dc.date.accessioned | 2020-09-24T08:00:47Z | |
dc.date.available | 2020-09-24T08:00:47Z | |
dc.date.issued | 2019-01 | |
dc.description.abstract | Objective: To develop and validate a new simple, accurate, precise and sensitive high performance liquid chromatographic method (HPLC) method for simultaneous estimation of ubidecarenone and vitamin E acetate in capsule dosage form as per international conference on harmonization (ICH) guidelines. Methods: The chromatographic separation of drugs were achieved using hypersil C8 column (250 mm x 4.6 mm, 5µ) in isocratic elution mode with a mobile phase of methanol: ethanol: n-hexane (80:10:10 v/v/v) at a flow rate of 1 ml/min with ultra-violet (UV) detection at 210 nm. Results: The optimized method produced sharp peaks with good resolution, minimum tailing factor and satisfactory retention time were found to be 5.745 min and 12.565 min for vitamin E acetate and ubidecarenone respectively. The method was linear in the range of 60-180 µg/ml for ubidecarenone and 20-60 µg/ml for vitamin E acetate with a correlation coefficient of 0.999 and 0.9993 respectively. Mean recoveries observed for ubidecarenone and vitamin E acetate were 99.85% and 99.73% respectively. The percentage relative standard deviation (% RSD) of peak area for system precision, method precision, and intermediate precision were found to be less than 0.37%. The lower degree of % RSD obtained has proved that the method was precise and robust. Conclusion: A new simple HPLC method was developed and validated as per ICH guidelines for the simultaneous estimation of ubidecarenone and vitamin E acetate and the method can be effectively applied for the routine analysis of active pharmaceutical ingredient (API) and formulations. | en_US |
dc.identifier.affiliations | Department of Pharmaceutical Analysis, Nandha college of Pharmacy, Erode-638 052, Tamilnadu, India | en_US |
dc.identifier.affiliations | Department of Pharmaceutical Analysis, Nandha college of Pharmacy, Erode-638 052, Tamilnadu, India | en_US |
dc.identifier.affiliations | Department of Pharmaceutical Analysis, Nandha college of Pharmacy, Erode-638 052, Tamilnadu, India | en_US |
dc.identifier.affiliations | Department of Pharmaceutical Chemistry, Nandha college of Pharmacy, Erode-638 052, Tamilnadu, India | en_US |
dc.identifier.affiliations | Department of Pharmaceutical Chemistry, Nandha college of Pharmacy, Erode-638 052, Tamilnadu, India | en_US |
dc.identifier.citation | Grace A. C., Prabha T., Jagadeeswaran M., Srinivasan K., Sivakumar T.. Analytical Method Development For Simultaneous Determination Of Ubidecarenone And Vitamin E Acetate In Capsule Dosage Form By Hplc. International Journal of Pharmacy and Pharmaceutical Sciences. 2019 Jan; 11(1): 79-84 | en_US |
dc.identifier.issn | 0975-1491 | |
dc.identifier.issn | 2656-0097 | |
dc.identifier.place | India | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/205809 | |
dc.language | en | en_US |
dc.publisher | Innovare Academic Sciences Pvt. Ltd. | en_US |
dc.relation.issuenumber | 1 | en_US |
dc.relation.volume | 11 | en_US |
dc.source.uri | https://doi.org/10.22159/ijpps.2019v11i1.30054 | en_US |
dc.subject | Ubidecarenone | en_US |
dc.subject | Vitamin E acetate | en_US |
dc.subject | HPLC | en_US |
dc.subject | Method development | en_US |
dc.subject | Coenzyme-Q10 | en_US |
dc.subject | alpha-tocopheryl acetate | en_US |
dc.title | Analytical Method Development For Simultaneous Determination Of Ubidecarenone And Vitamin E Acetate In Capsule Dosage Form By Hplc | en_US |
dc.type | Journal Article | en_US |
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