Anticataract activity of ethanolic extract of nigella sativa on glucose induced cataract in Goat Eye Lens.
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Date
2011-10
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Abstract
The present investigation was aimed to evaluate efficacy of ethanolic extract seeds of
Nigella sativa against glucose induced cataract in goat eye lens. In the in vitro study, goat lenses were
subjected to photographic evaluation and biochemical parameters such as protein, GSH, MDA,
NA+/K+ ATPase and sodium and potassium were also analyzed. Photographic examination of the eyes
showed that treatment with ethanolic extracts of seeds of N. sativa retarded the progression of lens
opacification. Cataract lens treated with N. sativa elevated the activity of Na+ K+ ATPase, total and
water soluble proteins and K+ ions to the level of normal level whereas reduced concentrations of Na+
ions. The MDA levels were significantly less in the N. sativa treated groups whereas, the level of
GSH, in high glucose (55mM), compared to the normal control group was significantly low but N.
sativa treated groups showed higher level of GSH. These results support the view that ethanolic
extract of seeds of N. sativa as seen in this in vitro model may, counteracts the effects of glucose in
inducing cataract to some extent.
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Keywords
Anticataract, Nigella sativa, goat lens, in vitro
Citation
Ahmed N Shabeer, Ahmed I Niyas, Waheed M P Ashar, Ali H Abdul Jaffar. Anticataract activity of ethanolic extract of nigella sativa on glucose induced cataract in Goat Eye Lens. International Journal of Applied Biology and Pharmaceutical Technology. 2011 Oct-Dec; 2(4): 274-279.