Adventitious Bud Formation and Plantlet Regeneration of Achyrocline satureoides-A Multipurpose Medicinal Plant.
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Date
2014-10
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Abstract
Adventitious bud regeneration was achieved from hypocotyls, cotyledons and leaf explants
of Achyrocline satureioides. Organogenesis was induced from every explant cultured on
Murashige and Skoog semisolid medium (plus sucrose 30g·L-1) containing different
combinations of 6-benzyladenine (BA) and -naphtalenacetic acid (NAA) under
116mol·m2·s-1 photosynthetic photon flux density (PPFD), photoperiod 14 h and at 272ºC.
The regeneration was similar for every tested explant and varied between 64 and 83%. The
number of buds formed per regenerative explants was similar in every treatment (5-8
shoots/explant). In order to stimulate In vitro rooting, regenerative leaves were sub cultured
from the best induction medium in MS lacking plant growth regulators for the same periods.
Every plantlet raised In vitro was phenotypically normal and successfully hardened to ex
vitro conditions. An experimental field plot with 60-day-old in vitro regenerated plants was
established.
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Keywords
Achyrocline satureoides, regeneration, leaf explants, cotyledon explants
Citation
Kotik D, Sansberro P, Luna C. Adventitious Bud Formation and Plantlet Regeneration of Achyrocline satureoides-A Multipurpose Medicinal Plant. European Journal of Medicinal Plants. 2014 Oct; 4(10): 1200-1209.