Toxicological Study of Leaf Extracts of Loranthus micranthus Linn Using Albino Wistar Rats

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dc.contributor.authorNnenna, Ani Onuabuchien_US
dc.contributor.authorOkwudili, Anien_US
dc.contributor.authorNwachukwu, Okwuosa Chukwugozieen_US
dc.date.accessioned2020-05-05T07:04:50Z
dc.date.available2020-05-05T07:04:50Z
dc.date.issued2020-04
dc.description.abstractThis study was aimed to investigate the effect of high sub-chronic doses of the aqueous and methanol leaf extracts of Loranthus micranthuson biochemical parameters of albino rats. Acute toxicity studies were performed according to standard methods. The animals were divided into 5 groups (n = 5). Aqueous and methanol extracts of L. micranthus leaves were administered in doses of 1000 and 2000 mg/kg body weight to four groups of rats respectively for 30 days through the intraperitoneal route. The fifth group served as control and received saline (5 ml/kg b.w, i.p). Blood samples were collected by retrorbital puncture and analyzed for biochemical and haematological parameters using assay kits. Acute toxicity studies indicated that both extracts had an LD50> 5000mg/kg. The results indicated significant (p<0.001) increases in alkaline phosphatase serum levels in both extract treated groups. The extracts also produced significant elevation in serum bilirubin levels when compared with normal control (p<0.05). Both extracts did not affect the levels of alanine and aspartate transaminases significantly (p>0.05). There were significant increase in the serum levels of urea in the extracts treated rats (p<0.05; p<0.01). The 2000 mg/kg aqueous extract produced significant increases in mean serum chloride and bicarbonate levels of treated rats when compared with control (p<0.01). The extracts produced significant decrease in the serum creatine kinase levels of treated rats in a non-dose related manner when compared with control (p<0.05). There were no significant changes in sodium and potassium levels of treated rats. The methanol extract had no significant effect on the haematological indices studied. The aqueous extract produced significant reductions in the haemoglobin and PCV of treated rats (p< 0.01). The total and differential leucocyte counts were not affected by extract treatment (p>0.05). From these results, the extracts caused significant biochemical changes but were not cytotoxic to leucocyte cell lines. Therefore, there should be caution in the long term use of these extractsen_US
dc.identifier.affiliationsDepartment of Applied Biochemistry, Faculty of Applied Natural Sciences, Enugu State University of Science and Technology, Enugu, Nigeria.en_US
dc.identifier.affiliationsDepartment of Medical Laboratory Sciences, Faculty of Health Science and Technology, University of Nigeria, Enugu Campus, Nigeria.en_US
dc.identifier.affiliationsDepartment of Medical Laboratory Sciences, Faculty of Health Science and Technology, University of Nigeria, Enugu Campus, Nigeria.en_US
dc.identifier.citationNnenna Ani Onuabuchi, Okwudili Ani, Nwachukwu Okwuosa Chukwugozie. Toxicological Study of Leaf Extracts of Loranthus micranthus Linn Using Albino Wistar Rats. International Journal of Biochemistry Research & Review. 2020 Apr; 29(3): 9-16en_US
dc.identifier.issn2231-086X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/200772
dc.languageenen_US
dc.publisherScience Domain Internationalen_US
dc.relation.issuenumber3en_US
dc.relation.volume29en_US
dc.source.urihttps://doi.org/10.9734/IJBCRR/2020/v29i330175en_US
dc.subjectNnennaen_US
dc.subjectAni Onuabuchien_US
dc.subjectOkwudilien_US
dc.subjectAnien_US
dc.subjectNwachukwuen_US
dc.subjectOkwuosa Chukwugozieen_US
dc.titleToxicological Study of Leaf Extracts of Loranthus micranthus Linn Using Albino Wistar Ratsen_US
dc.typeJournal Articleen_US
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