A study on cryopreservation of cultured rabbit periodontal ligament cells.

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dc.contributor.authorPal, Tamal Ken_US
dc.contributor.authorAibara, Farzan Jen_US
dc.contributor.authorAli, Nahiden_US
dc.date.accessioned2009-05-28T07:55:52Z
dc.date.available2009-05-28T07:55:52Z
dc.date.issued2002-01-08en_US
dc.description.abstractCultured rabbit periodontal ligament cells were subjected to short term cryopreservation in liquid nitrogen, for a period of 84 hours and 168 hours, to study the effect of cryopreservation on coll viability and culturing ability. The vital cell count performed by Trypan Blue exclusion was 14.93 x 10(0) cells/ml in vial A and 9.11 x 10(6) cell/ml in vial B, before cryopreservation. The loss of viability was minimal--vital cell count being 14.64 x 10(6) cells/ml in vial A and 8.87 x 10(6) cells/ml in vial B after 84 hrs of cryopreservation and 14.6 x 10(6) cells/ml in vial A and 8.82 x 10(6) cells/ml in vial B after 168 hours of cryopreservation. The cryopreserved cells after thawing could grow again in cultured.en_US
dc.description.affiliationDepartment of Periodontics, Dr. R. Ahmed Dental College, Kolkata, India. tamalpai@cal2.vsnl.net.inen_US
dc.identifier.citationPal TK, Aibara FJ, Ali N. A study on cryopreservation of cultured rabbit periodontal ligament cells. Indian Journal of Dental Research. 2002 Jan-Mar; 13(1): 55-63en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/51886
dc.language.isoengen_US
dc.source.urihttps://www.ijdr.inen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Adhesionen_US
dc.subject.meshCell Counten_US
dc.subject.meshCell Divisionen_US
dc.subject.meshCell Survivalen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshColoring Agents --diagnostic useen_US
dc.subject.meshCryopreservationen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshFemaleen_US
dc.subject.meshFibroblasts --cytologyen_US
dc.subject.meshMaleen_US
dc.subject.meshNitrogenen_US
dc.subject.meshPeriodontal Ligament --cytologyen_US
dc.subject.meshRabbitsen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTrypan Blue --diagnostic useen_US
dc.titleA study on cryopreservation of cultured rabbit periodontal ligament cells.en_US
dc.typeJournal Articleen_US
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