Characterization of oleic acid-induced acute respiratory distress syndrome model in rat.

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dc.contributor.authorAkella, Aparna
dc.contributor.authorSharma, Parul
dc.contributor.authorPandey, Ratna
dc.contributor.authorDeshpande, Shripad B
dc.date.accessioned2014-12-11T08:47:12Z
dc.date.available2014-12-11T08:47:12Z
dc.date.issued2014-07
dc.description.abstractAnimal studies using oleic acid (OA) model to produce acute respiratory distress syndrome (ARDS) have been inconsistent. Therefore, the present study was undertaken to establish an acute model of ARDS in rats using OA and to characterize its effect on cardio-respiratory parameters and lethality. The trachea, jugular vein and femoral artery of anesthetized adult rats were cannulated. A dose of OA (30-90 µL; iv) was injected in each animal and changes in respiratory frequency (RF), heart rate (HR) and mean arterial pressure (MAP) were recorded. Minute ventilation and PaO2/FiO2 (P/F) ratio were also determined. At the end, lungs were excised for determination of pulmonary water content and histological examination. At all doses of OA, there was immediate decrease followed by increase in RF, however at 75 and 90 µL of OA, RF decreased abruptly and the animals died by 63 ± 8.2 min and 19 ± 6.3 min; respectively. In all the groups, HR and MAP changes followed the respiratory changes. The minute ventilation increased in a dose-dependent manner while the values of P/F ratio decreased correspondingly. Pulmonary edema was induced at all doses. Histological examination of the lung showed alveolar damage, microvascular congestion, microvascular injury, infiltration of inflammatory cells, pulmonary edema and necrosis in a dose-dependent manner. With these results, OA can be used to induce different grades of ARDS in rats and OA doses of 50, 60 and 75 µL resemble mild, moderate and severe forms of ARDS respectively. Hence, OA model serves as a useful tool to study the pathophysiology of ARDS.en_US
dc.identifier.citationAkella Aparna, Sharma Parul, Pandey Ratna, Deshpande Shripad B. Characterization of oleic acid-induced acute respiratory distress syndrome model in rat. Indian Journal of Experimental Biology. 2014 Jul; 52(7): 712-719.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/153751
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/29044en_US
dc.subjectAcute lung injury (ALI)en_US
dc.subjectMinute ventilationen_US
dc.subjectP/F ratioen_US
dc.subjectPulmonary edemaen_US
dc.subjectSurfactanten_US
dc.subject.meshAnimals
dc.subject.meshCardiovascular Physiological Phenomena --drug effects
dc.subject.meshDisease Models, Animal
dc.subject.meshFemale
dc.subject.meshHeart Rate --drug effects
dc.subject.meshInflammation --chemically induced
dc.subject.meshInflammation --mortality
dc.subject.meshInflammation --pathology
dc.subject.meshMale
dc.subject.meshNecrosis
dc.subject.meshOleic Acid --toxicity
dc.subject.meshPulmonary Edema --chemically induced
dc.subject.meshPulmonary Edema --mortality
dc.subject.meshPulmonary Edema --pathology
dc.subject.meshPulmonary Ventilation --drug effects
dc.subject.meshRats
dc.subject.meshRespiratory Distress Syndrome, Adult --chemically induced
dc.subject.meshRespiratory Distress Syndrome, Adult --mortality
dc.subject.meshRespiratory Distress Syndrome, Adult --pathology
dc.subject.meshRespiratory Rate --drug effects
dc.subject.meshSurvival Rate
dc.titleCharacterization of oleic acid-induced acute respiratory distress syndrome model in rat.en_US
dc.typeArticleen_US
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