In vitro regeneration in olive (Olea europaea L.) cv, ‘Frontio’ from nodal segments.

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dc.contributor.authorMangal, Manisha
dc.contributor.authorSharma, Dheeraj
dc.contributor.authorSharma, Mamta
dc.contributor.authorOlea, europaea
dc.date.accessioned2014-12-12T05:32:42Z
dc.date.available2014-12-12T05:32:42Z
dc.date.issued2014-09
dc.description.abstractAn efficient and reproducible protocol for plantlet regeneration from nodal segments of Olive cv ‘Frontio’ has been developed. Media and explants browning due to exudation of phenolics from the explants were controlled by fortification of the medium with 100 mg/L ascorbic acid. Best establishment of olive explants was observed on half-strength MS salts fortified with 2.0 mg/L 6-benzylaminopurine (BAP), which resulted in 56.2% of bud break and 93.7% survival whereas, a combination of full strength MS medium with 1.0 mg/L each of 3-indole-butyric-acid (IBA) and kinetin was found to be the best for shoot multiplication, in terms of number of shoots (3.6 shoots/explant) and shoot length (2.2 cm). The in vitro shoots were rooted on half-strength MS medium fortified with 0.2 mg/L IBA and 0.2 mg/L α-naphthalene acetic acid (NAA) with 1.5 g/L activated charcoal, which supported optimum rooting (60 %), with an average of 2-3 roots/shoot, about 2.4 cm length were produced on four weeks of culture.en_US
dc.identifier.citationMangal Manisha, Sharma Dheeraj, Sharma Mamta, Kumar Sunil. In vitro regeneration in olive (Olea europaea L.) cv, ‘Frontio’ from nodal segments. Indian Journal of Experimental Biology. 2014 Sept; 52(9): 912-916.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/153776
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/29338en_US
dc.subjectMicropropagationen_US
dc.subjectNodal segmenten_US
dc.subjectOlea europaeaen_US
dc.subject.meshAntioxidants --pharmacology
dc.subject.meshAscorbic Acid --pharmacology
dc.subject.meshOlea --drug effects
dc.subject.meshOlea --physiology
dc.subject.meshPlant Roots --drug effects
dc.subject.meshPlant Roots --physiology
dc.subject.meshPlant Shoots --drug effects
dc.subject.meshPlant Shoots --physiology
dc.subject.meshRegeneration --drug effects
dc.titleIn vitro regeneration in olive (Olea europaea L.) cv, ‘Frontio’ from nodal segments.en_US
dc.typeArticleen_US
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