Evaluation of antibiotic resistance and quorum sensing assay of Enterococcus faecalis isolates from the urine samples

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dc.contributor.authorSagar, AJen_US
dc.contributor.authorOli, AKen_US
dc.contributor.authorJain, Aen_US
dc.contributor.authorChandrakanth, RK.en_US
dc.date.accessioned2024-11-30T07:55:52Z
dc.date.available2024-11-30T07:55:52Z
dc.date.issued2024-05
dc.description.abstractThe Gram-positive Enterococci found in the normal flora of the gastrointestinal tract (GIT) and urinary tract infection (UTI) were characterized by high resistance to antibiotics and large virulence. The aim of our study was to evaluate the diversity of antibiotic-resistant and quorum-sensing assays of Enterococcus faecalis isolated from urine samples of children aged between 11 and 15 with UTI. A total of 155 samples were collected from hospitals and diagnostic centers of Kalaburagi region. The E. faecalis isolates were screened on bile esculin azide agar media. Antibiotic susceptibility test was performed using different antibiotics as per Clinical and Laboratory Standards Institute guidelines. Minimum inhibitory concentrations (MICs) for gentamycin and vancomycin were determined by the method of broth dilution. Quorum sensing assay of E. faecalis was determined by a bioluminescence-based method. The species-level identification of E. faecalis isolates was determined by 16S rRNA polymerase chain reaction amplification. The incidence of E. faecalis in urine samples was found to be 47.74% (74/155). Antimicrobial susceptibility test (AST) indicates that 27% of isolates were multi-drug resistant, showing increased resistance to gentamycin and vancomycin (54.05%). MIC results show that 23 isolates were resistant to gentamycin with concentration of 128 ?g/ml and 17 isolates were resistant to vancomycin with concentration of 1024 ?g/ml. The quorum sensing assay has shown that the normalized relative light units range from 0.4 to 1.2. According to the 16srRNA gene amplification, out of 10 isolates, seven were identified as Enterococcus faecalis and three isolates were Streptococcus gallolyticus. This study emphasizes the development of increased vancomycin resistance of E. faecalis and indicates the presence of the virulence factors through the autoinducer assay. It is been suggested that major precaution measures should be implemented in the eradication of VRE in UTI infection.en_US
dc.identifier.affiliationsDepartment of Biotechnology, Gulbarga University, Kalaburagi, Karnataka, Indiaen_US
dc.identifier.affiliationsSDM Research Institute for Biomedical Sciences, a Constituent of Shri Dharmasthala Manjunatheshwara University, 5th Floor, Manjushree Building, SDMCMSH campus, Sattur, Dharwad, Karnataka, Indiaen_US
dc.identifier.affiliationsSDM Research Institute for Biomedical Sciences, a Constituent of Shri Dharmasthala Manjunatheshwara University, 5th Floor, Manjushree Building, SDMCMSH campus, Sattur, Dharwad, Karnataka, Indiaen_US
dc.identifier.affiliationsDepartment of Biotechnology, Gulbarga University, Kalaburagi, Karnataka, India.en_US
dc.identifier.citationSagar AJ, Oli AK, Jain A, Chandrakanth RK. . Evaluation of antibiotic resistance and quorum sensing assay of Enterococcus faecalis isolates from the urine samples . Journal of Applied Biology & Biotechnology. 2024 May; 12(3): 88-94en_US
dc.identifier.issn2489-7005
dc.identifier.issn2347-212X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/236739
dc.languageenen_US
dc.publisherOpen Science Publishers LLPen_US
dc.relation.issuenumber3en_US
dc.relation.volume12en_US
dc.source.urihttps://doi.org/10.7324/JABB.2024.147801en_US
dc.subjectAntibiotic resistanceen_US
dc.subjectEnterococcus faecalisen_US
dc.subjectQuorum sensingen_US
dc.subjectPolymerase chain reactionen_US
dc.subjectVancomycin-resistant enterococci.en_US
dc.titleEvaluation of antibiotic resistance and quorum sensing assay of Enterococcus faecalis isolates from the urine samplesen_US
dc.typeJournal Articleen_US
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