Influence of genotypes, growth regulators and basal media on direct differentiation of shoot buds from leaf segments of marigold (Tagetes spp.)

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dc.contributor.authorKumar, K Ravindraen_US
dc.contributor.authorSingh, Kanwar Palen_US
dc.contributor.authorRaju, DVSen_US
dc.contributor.authorBhatia, Reetaen_US
dc.contributor.authorPanwar, Sapnaen_US
dc.date.accessioned2020-01-02T06:38:40Z
dc.date.available2020-01-02T06:38:40Z
dc.date.issued2019-01
dc.description.abstractMarigold is a commercially important flower crop popular for its multipurpose use as loose flower, ornamental pot plant, pharmaceutical and industrial crop for lutein pigment extraction. The genus Tagetes is considered as a recalcitrant in terms of in vitro morphogenesis and plant regeneration. In the present study, we propose an efficient protocol for direct differentiation of shoot buds from immature leaf segments of African (Tagetes erecta L.) and French marigold (Tagetes patula L.) genotypes viz., Pusa Basanti Gainda and Pusa Arpita, respectively. We developed this protocol as follows. Leaf segments were collected from in vitro proliferated shoots established in aseptic culture from shoot tips of doubled haploids and dihaploids. Rapid and direct shoot bud induction was observed on enriched MS (EMS) medium supplemented with coconut water, AgNO3, casein hydrolysate, polyvinylpyrrolidone and glutamine. Among the different treatments, the highest (89.7%) direct shoot bud induction, bud forming capacity index (BFC) (2.36) and rapid (14 days) organogenesis was observed from leaf segments of French marigold cv. Pusa Arpita cultured on EMS media supplemented with 2.0 mg L-1 6-benzylaminopurine (BAP) and 0.5 mg L-1 1-naphthalene acetic acid (NAA). African marigold cv. Pusa Basanti Gainda exhibited maximum regeneration (66.6%), BFC index (2.11) and early (20 days) shoot bud induction on EMS media supplemented with 0.5 mg L-1 BAP and 0.25 mg L-1 NAA. In order to improve the regeneration capacity, different maturity groups of distal and proximal leaf ends were cultured on genotype specific medium. Among the different explant groups, immature leaf basal segments exhibited highest BFC index of 4.2 and 2.6 in Pusa Arpita and Pusa Basanti Gainda respectively. All the regenerated shoots were excised, rooted in 0.5 mg L-1 IBA and transplanted under polyhouse conditions, where they exhibit normal morphological growth similar to mother plant.en_US
dc.identifier.affiliationsDr. YSRHU, Horticultural Research Station, Kovvur-534 350, Andhra Pradesh, Indiaen_US
dc.identifier.affiliationsDivision of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, New Delhi-110 012, Indiaen_US
dc.identifier.affiliationsICAR-Directorate of Floriculture Research, Pune, Maharashtra, Indiaen_US
dc.identifier.affiliationsICAR-IARI Regional Station, Katrain 175 129, Himachal Pradesh, Indiaen_US
dc.identifier.citationKumar K Ravindra, Singh Kanwar Pal, Raju DVS, Bhatia Reeta, Panwar Sapna. Influence of genotypes, growth regulators and basal media on direct differentiation of shoot buds from leaf segments of marigold (Tagetes spp.). Indian Journal of Experimental Biology. 2019 Jan; 57(1): 30-39en_US
dc.identifier.issn0975-1009
dc.identifier.issn0019-5189
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/191461
dc.languageenen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.relation.issuenumber1en_US
dc.relation.volume57en_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/45565en_US
dc.titleInfluence of genotypes, growth regulators and basal media on direct differentiation of shoot buds from leaf segments of marigold (Tagetes spp.)en_US
dc.typeJournal Articleen_US
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