Molecular Detection of Mycological Content in Ready to Eat Garri in Bayelsa State
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Date
2020-05
Journal Title
Journal ISSN
Volume Title
Publisher
SCIENCEDOMAIN international
Abstract
Background and Objective: Garri is a powdery carbohydrate-based food material of cassava root tubers (Manihot esculenta Crantz) consumed predominantly in West African countries. It can be processed with palm oil rich in carotenoid (Light-yellow) or without palm oil (Creamy-white). In Nigeria, garri is widely acceptable and consumed by both the poor, the middle men or average Nigerian, and also the rich because it serves as a major source of carbohydrate.The study aimed at detecting fungal strains that produce mycotoxins in garri sourced from Southern Ijaw Local Government Area of Bayelsa State.Materials and Methods:A total number of fifty freshly prepared and market displayed-garri samples were collected and processed using standard mycological techniques and Polymerase Chain Reaction from the 23 villages that constitute the LGA.Results:Results showed that the moulds isolated in yellow garri samples in this study were; Aspergillus spp9(37.50%),Cladosporiumspp 5(20.83%),Fusarium spp4(16.67%),Penicillus spp3(12.50%),Rhizopus spp2(8.30%) and Mucor spp1(4.17%),while those isolated in the white garri samples were; Aspergillus spp 6(25%), Penicilliumspp 8(33.3%), Cladosporium spp 4(16.7%), Rhizopus spp 2(8.3%),Mucor spp 1(4.1%), Alternaria spp 2(8.3%) and Fusarium spp 1(4.1%) with predominance of Penicilliumand Asperigellus species. Twenty samples subjected to molecular analysis to determine the Internal Transcribed Spacerregion (ITS) and characterization of the fungal strains were all positive (100%).Conclusion:Fungal contamination on garri mostly results from unhealthy post-processing activity basically from poor packaging and storage. Mycotoxins from fungal strains have serious health implications on humans therefore it is paramount that proper packaging and storage of this product is publicized to reduce its mycological contamination.
Description
Keywords
Garri, cassava root tubers, molds, PCR, Bayelsa State.
Citation
Tolulope Alade, Itodo Sunday Ewaoche, Oluwayemisi Agnes Olorode, Deborah Levi. Molecular Detection of Mycological Content in Ready to Eat Garri in Bayelsa State. Journal of Advances in Medicine and Medical Research. 2020 May; 32(7): 64-72