Detection of hepatitis C virus RNA by polymerase chain reaction: Design of amplification primers.

Simple item page
dc.contributor.authorSongsivilai, Sirirurg
dc.contributor.authorLuangwedchakarn, Voravich
dc.contributor.authorKanistanon, Duangjit
dc.contributor.authorLouisirirotchanakul, Suda
dc.contributor.authorDharakul, Tararaj
dc.date.accessioned2012-04-27T10:32:53Z
dc.date.available2012-04-27T10:32:53Z
dc.date.issued1993-09
dc.description.abstractNew sets of primers for amplification of hepatitis C virus (HCV) RNA were designed from the conserved regions of American and Japanese isolates of HCV. Primers set A amplified parts of the 5’-untranslated and core gene regions, whereas set B amplified parts of the core and envelope gene regions. PCR amplification were carried out from HCV RNA isolated from sera of 13 Thai patients with antibody to HCV, using these newly developed primers. HCV RNA was detectable in 8 patients (61.5%). Of those PCR positive samples, only 4 patients (50%) were positive with primer set A, whereas 7 patients (87.5%) were positive with primer set B. Interestingly, 4 patients were tested positive with only set B, 3 patients with both sets A and B, and just one patient with set A only. This result suggests that PCR assay as a diagnostic tool for HCV may need to be carried out with more than one primer set. The relatively low percentage of PCR positivity of the HCV from Thai patients using primer set A, which was previously identified as the most conserved region of the HCV genome, also indicates that the sequence of the Thai isolates of the virus may be different from those of the American and Japanese strains.en_US
dc.identifier.citationSongsivilai Sirirurg, Luangwedchakarn Voravich, Kanistanon Duangjit, Louisirirotchanakul Suda, Dharakul Tararaj. Detection of hepatitis C virus RNA by polymerase chain reaction: Design of amplification primers. Siriraj Medical Journal, 1993 Sept; 45(9): 597-603.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/138009
dc.language.isoenen_US
dc.source.urihttps://www.sirirajmedj.com/content_download.php?content_id=1707en_US
dc.subjectHepatitis C virus RNAen_US
dc.subjectPolymerase chain reactionen_US
dc.titleDetection of hepatitis C virus RNA by polymerase chain reaction: Design of amplification primers.en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
smj1993v45n9p597.pdf
Size:
1006.71 KB
Format:
Adobe Portable Document Format
Description:
Journal article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Siriraj Medical Journal