Determination of subgroup, serotype and electropherotype of human rotaviruses obtained from diarrheic patients in the Philippines.

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dc.contributor.authorLintag, I Cen_US
dc.contributor.authorKobayashi, Nen_US
dc.contributor.authorUrasawa, Ten_US
dc.contributor.authorTaniguchi, Ken_US
dc.contributor.authorSaniel, M Cen_US
dc.contributor.authorUrasawa, Sen_US
dc.date.accessioned2009-05-27T16:42:41Z
dc.date.available2009-05-27T16:42:41Z
dc.date.issued1990-06-01en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractA total of 40 rotavirus specimens obtained from infants with acute gastroenteritis in Metro Manila, Philippines, were analyzed for their subgroup and serotype specificities and RNA electropherotype. A half (50%) of the specimens contained subgroup I human rotaviruses (HRVs), while 45% of them contained subgroup II HRVs. All subgroup II HRVs were determined to be serotype 1, except one specimen belonging to serotype 3, by an enzyme-linked immunosorbent assay with serotype specific monoclonal antibodies. However, serotype specificity of all subgroup I specimens failed to be determined. In polyacrylamide gel electrophoresis, RNAs extracted from HRVs of either subgroup showed long electropherotype. At least two electropherotypes were distinguished in subgroup I viruses, while five different patterns of viral RNA were found in subgroup II viruses.en_US
dc.description.affiliationResearch Institute for Tropical Medicine, Muntinlupa, Metro Manila, Philippines.en_US
dc.identifier.citationLintag IC, Kobayashi N, Urasawa T, Taniguchi K, Saniel MC, Urasawa S. Determination of subgroup, serotype and electropherotype of human rotaviruses obtained from diarrheic patients in the Philippines. The Southeast Asian Journal of Tropical Medicine and Public Health. 1990 Jun; 21(2): 249-58en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/35837
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshElectrophoresis, Gel, Two-Dimensionalen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFemaleen_US
dc.subject.meshGastroenteritis --epidemiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshInfanten_US
dc.subject.meshInfant, Newbornen_US
dc.subject.meshMaleen_US
dc.subject.meshPhilippines --epidemiologyen_US
dc.subject.meshRNA --analysisen_US
dc.subject.meshRotavirus --classificationen_US
dc.subject.meshRotavirus Infections --epidemiologyen_US
dc.subject.meshSeroepidemiologic Studiesen_US
dc.subject.meshSerotypingen_US
dc.titleDetermination of subgroup, serotype and electropherotype of human rotaviruses obtained from diarrheic patients in the Philippines.en_US
dc.typeJournal Articleen_US
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