Determination of subgroup, serotype and electropherotype of human rotaviruses obtained from diarrheic patients in the Philippines.
No Thumbnail Available
Date
1990-06-01
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
A total of 40 rotavirus specimens obtained from infants with acute gastroenteritis in Metro Manila, Philippines, were analyzed for their subgroup and serotype specificities and RNA electropherotype. A half (50%) of the specimens contained subgroup I human rotaviruses (HRVs), while 45% of them contained subgroup II HRVs. All subgroup II HRVs were determined to be serotype 1, except one specimen belonging to serotype 3, by an enzyme-linked immunosorbent assay with serotype specific monoclonal antibodies. However, serotype specificity of all subgroup I specimens failed to be determined. In polyacrylamide gel electrophoresis, RNAs extracted from HRVs of either subgroup showed long electropherotype. At least two electropherotypes were distinguished in subgroup I viruses, while five different patterns of viral RNA were found in subgroup II viruses.
Description
The Southeast Asian Journal of Tropical Medicine and Public Health.
Keywords
Citation
Lintag IC, Kobayashi N, Urasawa T, Taniguchi K, Saniel MC, Urasawa S. Determination of subgroup, serotype and electropherotype of human rotaviruses obtained from diarrheic patients in the Philippines. The Southeast Asian Journal of Tropical Medicine and Public Health. 1990 Jun; 21(2): 249-58