Study of biofilm formation and its correlation with highly drug resistant uropathogens in catheter-associated urinary tract infection

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dc.contributor.authorQayoom, Jalilaen_US
dc.contributor.authorHaswani, Nehaen_US
dc.contributor.authorPai, Vidyaen_US
dc.date.accessioned2020-11-18T10:23:44Z
dc.date.available2020-11-18T10:23:44Z
dc.date.issued2018-07
dc.description.abstractAims and objectives: 1. To put culture and sensitivity of urine samples from catheterised patients withsymptomatic and asymptomatic bacteriuria. 2. To detect the biofilm formation by tube adherence and tissueculture plate method. 3. To compare the antibiotic sensitivity between biofilm and non-biofilm formingorganisms. Materials and Methods: The study was conducted in the Department of Microbiology of YenepoyaMedical College and Hospital, Mangalore, India. A total of 100 bacterial isolates obtained from urine samples ofcatheterised patients were included in the study. The processing of the samples (culture and sensitivity) weredone according to standard microbiological techniques. The biofilm formation was done by tube adherence andtissue culture plate method. Results: Out of 100 uropathogens isolated, 96(96%) were Gram negative bacilli.Four isolates were Gram positive cocci. Amongst the Gram negative bacilli, E.coli were 69%, Klebsiellapneumoniae 19%, Acinetobacter species 5% and Pseudomonas aeruginosa 3%. All gram positive cocci wereEnterococci (4%). In the tube adherence method, the number of isolates showing biofilm formation was 51%and non-biofilm producers was 49%. By tissue culture plate method, the number of isolates showing biofilmformation was 63% and non-biofilm producers was 37%.The predominant organism showing biofilm formationwere K.pneumoniae(89%) followed by Acinetobacter spp. (40%).There was significant correlation betweenbiofilm formation and multi-drug resistance. Conclusion: Significant correlation between biofilm productionand multi-drug resistance was observed in our study. The study will help the clinician to take a call on nonresponding uropathogens and decide on better therapeutic options amongst those available in CAUTIs.en_US
dc.identifier.affiliationsPost-graduate student, Department of Microbiology, Yenepoya Medical College and Hospital, Deralakatte, Mangalore- 575008, Karnataka, Indiaen_US
dc.identifier.affiliationsAssistant Professor, Department of Microbiology, Yenepoya Medical College and Hospital, Deralakatte, Mangalore, India, dr.nehahaswani@gmail.comen_US
dc.identifier.affiliationsProfessor & Head, Department of Microbiology, Yenepoya Medical College and Hospital, Deralakatte, Mangalore, Indiaen_US
dc.identifier.citationQayoom Jalila, Haswani Neha, Pai Vidya. Study of biofilm formation and its correlation with highly drug resistant uropathogens in catheter-associated urinary tract infection. Journal of International Medicine and Dentistry. 2018 Jul; 5(2): 87-92en_US
dc.identifier.issn2454-8847
dc.identifier.issn2350-045X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/215581
dc.languageenen_US
dc.publisherVXL Publishersen_US
dc.relation.issuenumber2en_US
dc.relation.volume5en_US
dc.source.urihttps://jimd.in/uploaded/volumes/Biofilm_formation_with_HDRU_in_CAUTI.pdfen_US
dc.subjectCAUTIen_US
dc.subjecttube adherence methoden_US
dc.subjecttissue culture plate methoden_US
dc.subjectHDRUen_US
dc.titleStudy of biofilm formation and its correlation with highly drug resistant uropathogens in catheter-associated urinary tract infectionen_US
dc.typeJournal Articleen_US
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