Human Papillomavirus Infection among Bolivian Amazonian Women.

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dc.contributor.authorLema,en_US
dc.contributor.authorHurtado,en_US
dc.contributor.authorSegurondo,en_US
dc.contributor.authorRomero,en_US
dc.contributor.authorDulon,en_US
dc.contributor.authorAsturizaga,en_US
dc.contributor.authorPanoso,en_US
dc.contributor.authorGarcia,en_US
dc.contributor.authorFujiyoshi,en_US
dc.contributor.authorYashiki,en_US
dc.contributor.authorLi,en_US
dc.contributor.authorLou,en_US
dc.contributor.authorCervantes,en_US
dc.contributor.authorGomez,en_US
dc.contributor.authorSonoda,en_US
dc.date.accessioned2009-05-27T17:52:07Z
dc.date.available2009-05-27T17:52:07Z
dc.date.issued2001-01-30en_US
dc.descriptionAsian Pacific Journal of Cancer Prevention.en_US
dc.description.abstractCervical cancer is the most common malignancy among women in Latin America. Human papilloma virus infection is known to be an important risk factor. However, HPV infection among Bolivian women has not yet been fully evaluated. The present study aimed to investigate HPV infection among women living in a rural region of the Bolivian Amazon. Cervical swab samples were collected from 151 healthy women in three Amazonian villages. From every woman, two samples were collected by cotton swab; one for cytological examination and the other for ethanol-preservation of cervical epithelial cells for HPV DNA testing. High molecular DNA was extracted from the ethanol-preserved cervical epithelial cells and tested for HPV DNA by a PCR-RFLP protocol. Ethanol-preserved cervical epithelial cells remained suitable for DNA isolation and PCR amplification of human b-globin and HPV E6/E7 genes, 25 days after sample collection in the field. HPV-31, HPV-58 and HPV-6 were detected in the studied population. The overall prevalence of HPV infection among Bolivian Amazonian women was 8.0%. Neither dual nor multiple HPV infections were found in any of the positive samples. This is the first report of HPV prevalence and type distribution among Bolivian Amazonian women. Our new method for preservation of cervical epithelial cells in ethanol may be useful for viro-epidemiological studies in rural areas.en_US
dc.description.affiliationDepartment of Virology, Faculty of Medicine, Kagoshima University, Kagoshima 890-8520, Japan. sonoda1@m2.kufm.kagoshima-u.ac.jpen_US
dc.identifier.citationLema CH, Hurtado LV, Segurondo D, Romero F, Dulon A, Asturizaga D, Panoso W, Garcia G, Fujiyoshi T, Yashiki S, Li HC, Lou H, Cervantes J, Gomez LH, Sonoda S. Human Papillomavirus Infection among Bolivian Amazonian Women. Asian Pacific Journal of Cancer Prevention. 2001 Apr-Jun; 2(2): 135-141en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/38055
dc.language.isoENGen_US
dc.source.urihttps://www.apocp.orgen_US
dc.titleHuman Papillomavirus Infection among Bolivian Amazonian Women.en_US
dc.typeJOURNAL ARTICLEen_US
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