Determination of PSP Toxins in Moroccan Shellfish by MBA, HPLC and RBA Methods: Links to Causative Phytoplankton Alexandrium minutum

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dc.contributor.authorR., Abouabdellahen_US
dc.contributor.authorA., Bennounaen_US
dc.contributor.authorM- Y., Dechraoui-Botteinen_US
dc.contributor.authorJ., El Attaren_US
dc.contributor.authorM., Dellalen_US
dc.contributor.authorA., Mbarkien_US
dc.contributor.authorM., Alahyaneen_US
dc.contributor.authorS., Benbrahimen_US
dc.date.accessioned2020-05-05T07:03:58Z
dc.date.available2020-05-05T07:03:58Z
dc.date.issued2018-04
dc.description.abstractParalytic shellfish poisoning (PSP) toxins are secondary metabolites of the toxic species of phytoplankton. The consumption of shellfish accumulating these toxins can cause neurological symptoms and even death. Within the framework of the surveillance program of seafood safety along the Moroccan littoral environment established by National Institute of Fisheries Research (INRH), a study of PST was conducted from 2004 to 2016 in south Moroccan’s shellfish, mussels from south Agadir region and Razor Shell from Dakhla bay. The surveillance was carried out bi-monthly or weekly using the AOAC official method of analysis (AOAC 959.08) mouse bioassay (MBA). In parallel, monitoring of toxic phytoplankton in water was conducted. With the aim to determine the shellfish toxin profile, ion-pair high-performance liquid chromatography with post-column derivatisation and fluorescence detection (HPLC-FD) was performed. The Receptor Binding Assay (RBA) also was used for determination of total toxicity of PSP toxins in Agadir’s mussels.In both regions, the analysis of seawater revealed the presence of the toxic algae Alexandrium spp during toxics events. Along the coast of Agadir, PSP toxins in shellfish were associated with the presence of Alexandrium cf. minutum in seawater. These toxic events were widely distributed in time and space and mainly detected during the summer and fall seasons. In some samples concentrations exceeded the sanitary threshold (ST) of 800 ?g eq STX /kg. HPLC analysis revealed that Saxitoxin and Gonyautoxins dominated the toxin profile. The comparison between different methods showed a strong uphill (positive) linear relationship, with a coefficient correlation of r=0.79 between MBA and HPLC and r = 0.809 between MBA and RBAen_US
dc.identifier.affiliationsNational Institute of Fisheries Research, Aghsdis New Port, Agadir, Moroccoen_US
dc.identifier.affiliationsNational Institute of Fisheries Research, Aghsdis New Port, Agadir, Moroccoen_US
dc.identifier.affiliationsInternational Atomic Energy Agency, Environment Laboratories Monaco, 4a Quai Antoine 1st, 98000, Monacoen_US
dc.identifier.affiliationsNational Institute of Fisheries Research, Aghsdis New Port, Agadir, Moroccoen_US
dc.identifier.affiliationsNational Institute of Fisheries Research, Dakhla, Moroccoen_US
dc.identifier.affiliationsNational Institute of Fisheries Research, Aghsdis New Port, Agadir, Moroccoen_US
dc.identifier.affiliationsNational Institute of Fisheries Research, Aghsdis New Port, Agadir, Moroccoen_US
dc.identifier.affiliationsDepartment of SSMM, National Institute of Fisheries Research, Casablanca, Moroccoen_US
dc.identifier.citationR. Abouabdellah, A. Bennouna, M- Y. Dechraoui-Bottein, J. El Attar, M. Dellal, A. Mbarki, M. Alahyane, S. Benbrahim. Determination of PSP Toxins in Moroccan Shellfish by MBA, HPLC and RBA Methods: Links to Causative Phytoplankton Alexandrium minutum. International Journal of Biochemistry Research & Review. 2018 Apr; 21(2): 1-12en_US
dc.identifier.issn2231-086X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/200678
dc.languageenen_US
dc.publisherScience Domain Internationalen_US
dc.relation.issuenumber2en_US
dc.relation.volume21en_US
dc.source.urihttps://doi.org/10.9734/IJBCRR/2018/40032en_US
dc.subjectParalytic shellfish poisoningen_US
dc.subjectAlexandrium minutumen_US
dc.subjectMoroccoen_US
dc.subjectHPLCen_US
dc.subjectRBAen_US
dc.subjectMBAen_US
dc.titleDetermination of PSP Toxins in Moroccan Shellfish by MBA, HPLC and RBA Methods: Links to Causative Phytoplankton Alexandrium minutumen_US
dc.typeJournal Articleen_US
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