Comparison of staining of mitotic figures by haematoxylin and eosin-and crystal violet stains, in oral epithelial dysplasia and squamous cell carcinoma.

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dc.contributor.authorAnkle, Madhuri Ren_US
dc.contributor.authorKale, Alka Den_US
dc.contributor.authorCharantimath, Seemaen_US
dc.date.accessioned2009-05-28T07:51:29Z
dc.date.available2009-05-28T07:51:29Z
dc.date.issued2007-07-10en_US
dc.description.abstractMitosis of cells gives rise to tissue integrity. Defects during mitosis bring about abnormalities. Excessive proliferation of cells due to increased mitosis is one such outcome, which is the hallmark in precancer and cancer. The localization of proliferating cells or their precursors may not be obvious and easy. Establishing an easy way to distinguish these mitotic cells will help in grading and understanding their biological potential. Although immunohistochemistry is an advanced method in use, the cost and time factor makes it less feasible for many laboratories. Selective histochemical stains like toluidine blue, giemsa and crystal violet have been used in tissues including the developing brain, neural tissue and skin. AIM OF THE STUDY: 1) To compare the staining of mitotic cells in haematoxylin and eosin with that in crystal violet. 2) To compare the number of mitotic figures present in normal oral mucosa, epithelial dysplasia and oral squamous cell carcinoma in crystal violet-stained sections with that in H and E-stained sections. MATERIALS AND METHODS: Ten tissues of normal oral mucosa and 15 tissues each of oral epithelial dysplasia seen in tobacco-associated leukoplakia and squamous cell carcinoma were studied to evaluate the selectivity of 1% crystal violet for mitotic figures. The staining was compared with standard H and E staining. Statistical analysis was done using Mann-Whitney U test. RESULTS: A statistically significant increase in the mean mitotic count was observed in crystal violet-stained sections of epithelial dysplasia as compared to the H and E-stained sections (p=0.0327). A similar increase in the mitotic counts was noted in crystal violet-stained sections of oral squamous cell carcinoma as compared to the H and E-stained sections.(p=0.0443). No significant difference was found in the mitotic counts determined in dysplasia or carcinoma by either the crystal violet (p=0.4429) or the H and E-staining techniques (p=0.2717). CONCLUSION: One per cent crystal violet provides a definite advantage over the H and E-stained sections in selectively staining the mitotic figures.en_US
dc.description.affiliationDepartment of Oral Pathology and Microbiology, Institute of Dental Sciences, K.L.E.S's Academy of Higher Education and Research, Nehru Nagar, Belgaum - 590 010, India. madhuriankle@yahoo.co.inen_US
dc.identifier.citationAnkle MR, Kale AD, Charantimath S. Comparison of staining of mitotic figures by haematoxylin and eosin-and crystal violet stains, in oral epithelial dysplasia and squamous cell carcinoma. Indian Journal of Dental Research. 2007 Jul-Sep; 18(3): 101-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/51776
dc.language.isoengen_US
dc.source.urihttps://www.ijdr.inen_US
dc.subject.meshCarcinoma, Squamous Cell --pathologyen_US
dc.subject.meshColoring Agents --diagnostic useen_US
dc.subject.meshEosine Yellowish-(YS) --diagnostic useen_US
dc.subject.meshEpithelium --pathologyen_US
dc.subject.meshFluorescent Dyes --diagnostic useen_US
dc.subject.meshGentian Violet --diagnostic useen_US
dc.subject.meshHematoxylin --diagnostic useen_US
dc.subject.meshHumansen_US
dc.subject.meshLeukoplakia, Oral --pathologyen_US
dc.subject.meshMitosis --physiologyen_US
dc.subject.meshMitotic Indexen_US
dc.subject.meshMouth Mucosa --pathologyen_US
dc.subject.meshMouth Neoplasms --pathologyen_US
dc.subject.meshPrecancerous Conditions --pathologyen_US
dc.subject.meshStaining and Labeling --methodsen_US
dc.titleComparison of staining of mitotic figures by haematoxylin and eosin-and crystal violet stains, in oral epithelial dysplasia and squamous cell carcinoma.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
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