Interaction of T-DNA border sequences and Ti-plasmid vir functions of Agrobacterium results in differential single-stranded linear T-DNA molecule production and plant transformation.

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dc.contributor.authorDas, Sen_US
dc.contributor.authorBasu, Den_US
dc.contributor.authorMukherjee, Pen_US
dc.contributor.authorSen, S Ken_US
dc.date.accessioned2009-05-28T12:12:12Z
dc.date.available2009-05-28T12:12:12Z
dc.date.issued1991-11-01en_US
dc.description.abstractThe mechanism of Agrobacterium mediated genetic transformation of plants is dependent upon certain genetic function of the chromosome of the bacterium as well as on Ti-plasmid borne vir loci and the border sequences of T-DNA. The organisationally variable forms of the naturally occurring border sequences amongst Ti-plasmid types are differentially responsive to gene products of vir loci concerned with T-strand production. Additionally, the production of stable transformants is dependent upon vir gene products effective after T strands are produced. The interaction of border sequences from different strains of Agrobacterium with vir proteins encoded by various helper plasmids revealed that functional differences do exist amongst vir gene products contained in the type of helper plasmids used.en_US
dc.description.affiliationPlant Molecular & Cellular Genetics, Bose Institute, Calcutta, India.en_US
dc.identifier.citationDas S, Basu D, Mukherjee P, Sen SK. Interaction of T-DNA border sequences and Ti-plasmid vir functions of Agrobacterium results in differential single-stranded linear T-DNA molecule production and plant transformation. Indian Journal of Experimental Biology. 1991 Nov; 29(11): 991-1001en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/57762
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDNA, Bacterial --geneticsen_US
dc.subject.meshDNA, Single-Stranded --metabolismen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPlants, Toxicen_US
dc.subject.meshPlasmidsen_US
dc.subject.meshRhizobium --geneticsen_US
dc.subject.meshTobacco --geneticsen_US
dc.subject.meshTransformation, Geneticen_US
dc.titleInteraction of T-DNA border sequences and Ti-plasmid vir functions of Agrobacterium results in differential single-stranded linear T-DNA molecule production and plant transformation.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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