Biosynthesis and characterization of silver nanoparticles from cassia auriculata leaf extract and in vitro evaluation of antimicrobial activity.
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Date
2012-04
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Abstract
The synthesis of metals and nanoparticles is an expanding research area due to the potential
applications for the development of novel technologies. Biosynthesis of silver nanoparticles was investigated by
reducing sliver nitrate with Cassiaauriculataleaf extract at room temperature. The plant belongs to family
Ceasalpiniaceae and the plant is having promising medicinal properties for a wide range of human diseases. The
synthesized nanoparticles characterized by the UV-Vis spectroscopy, revealed the formation of silver nanoparticles
by exhibiting the typical surface plasmon absorption maxima at 420-435 nm.The peaks in the X ray Diffraction
pattern are in good agreement with the standard values of the face-centered-cubic form of metallic silver. Fourier
transform infrared spectroscopy indicates that the compounds attached with silver nanoparticles could be
polyphenols with aromatic ring and bound amide region and transmission electron microscope reveals that the
particles are spherical and polydispersed.The antimicrobial activity of synthesized nanoparticles were evaluated
against E.coli, Sarratiamarcascence, Bacillus subtilis ,Aspergillusniger and Aspergillusflavus. Fungi were most
susceptible to silver nanoparticles followed by bacteria.
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Keywords
Biosynthesis, Silver nanoparticles, Cassia auriculata, Ceasalpiniaceae, antimicrobial
Citation
Parveen Asra, Roy Aashis S, Rao Srinath. Biosynthesis and characterization of silver nanoparticles from cassia auriculata leaf extract and in vitro evaluation of antimicrobial activity. International Journal of Applied Biology and Pharmaceutical Technology. 2012 Apr-Jun; 3(2): 222-228.