Cell-free DNA in Embryo Culture Media as Non-invasive Biomarker of the Quality of Embryo Cleavage

Simple item page
dc.contributor.authorJamil, M.en_US
dc.contributor.authorDebbarh, H.en_US
dc.contributor.authorJelloul, H.en_US
dc.contributor.authorKabit, A.en_US
dc.contributor.authorEnnaji, M.en_US
dc.contributor.authorZarqaoui, M.en_US
dc.contributor.authorHissane, E. M.en_US
dc.contributor.authorSenhaji, W.en_US
dc.contributor.authorLouanjli, N.en_US
dc.contributor.authorCadi, R.en_US
dc.date.accessioned2023-07-14T08:03:58Z
dc.date.available2023-07-14T08:03:58Z
dc.date.issued2023-02
dc.description.abstractBackground: The success of in vitro-fertilization (IVF) cycles is determined in large part by the quality of embryo cleavage, which in turn, is dependent on the quality of the embryo culture media (CM). Many factors can influence the quality of embryo CM, one of which is the levels of Cell Free Deoxyribonucleic acid (DNA). Understanding the association between Cell-free DNA levels in embryo CM and the quality of embryo cleavage could help improve the quality of IVF techniques. Methods: This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. After intracytoplasmic sperm injection (ICSI), 48 embryos were evaluated on day 3 of their development, according to their cell number. Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. Results: The results revealed a significant increase in Cell-free DNA levels on day 2 CM corresponding to 4 to 6 cell embryos compared to those corresponding to 7 to 8 cell embryos (p=0.04). As for day 3 CM, the results showed no significant difference between the Cell-Free DNA levels in CM of 7-8 and those of 4-6 cell embryos (p=0.4). Also, cell free DNA levels in embryo CM, were significantly higher on day 2 compared to day 3 for both 7-8 and 4-6 cell embryos (p=0.03; p=0.04). Conclusion: We conclude that cell-free DNA levels in CM might be associated with delayed embryo cleavage.en_US
dc.identifier.affiliationsDepartment of Biology, Laboratory of Molecular Genetic Physiopathology and Biotechnology, Ain Chock Faculty of Sciences, Hassan II University, Casablanca, Moroccoen_US
dc.identifier.affiliationsIVF Center IRIFIV, Iris Clinic, Casablanca, Moroccoen_US
dc.identifier.citationJamil M., Debbarh H., Jelloul H., Kabit A., Ennaji M., Zarqaoui M., Hissane E. M., Senhaji W., Louanjli N., Cadi R.. Cell-free DNA in Embryo Culture Media as Non-invasive Biomarker of the Quality of Embryo Cleavage. Annual Research & Review in Biology. 2023 Feb; 38(2): 12-18en_US
dc.identifier.issn2347-565X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/219418
dc.languageenen_US
dc.publisherSciencedomain Internationalen_US
dc.relation.issuenumber2en_US
dc.relation.volume38en_US
dc.source.urihttps://doi.org/10.9734/arrb/2023/v38i230569en_US
dc.subjectCell-free DNAen_US
dc.subjectembryo cleavageen_US
dc.subjectembryo cultureen_US
dc.subjectin vitro fertilizationen_US
dc.titleCell-free DNA in Embryo Culture Media as Non-invasive Biomarker of the Quality of Embryo Cleavageen_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ARRB2023v38n2p12.pdf
Size:
723.13 KB
Format:
Adobe Portable Document Format
Download
Collections
Annual Research & Review in Biology