Studies on glucoamylase produced from Aspergillus awamori (NRRL-3112) and their effect on saccharification of potato starch.

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dc.contributor.authorKumar Soni, Sen_US
dc.contributor.authorVenkateswara Rao, Men_US
dc.contributor.authorDas, Den_US
dc.date.accessioned2009-05-28T12:47:53Z
dc.date.available2009-05-28T12:47:53Z
dc.date.issued1995-12-01en_US
dc.description.abstractEfficiency of an enzymatic starch saccharification process depends not only on the activity of the glucoamylase but also its purity. About 96.82% unwanted proteins present in 2-day old culture broth of A. awamori NRRL-3112 (grown in MYGP medium) were separated by precipitation with ammonium sulphate which was followed by dialysis. More than 80% activity of the glucoamylase was recovered. Three protein fractions (A, B, C) were identified using gel permeation chromatography. Fractions A and B showed comparatively higher glucoamylase activity than fraction-C. Moreover, fraction-B showed no product inhibition. The optimum temperature and pH of the purified enzyme (fraction-B) were 60 degrees C and 4.0 respectively. The saccharification efficiency of potato pulp was more in case of using purified glucoamylase (fraction-B) as compared to that of crude enzyme.en_US
dc.description.affiliationChemical Engineering Department, Indian Institute of Technology, Kharagpur.en_US
dc.identifier.citationKumar Soni S, Venkateswara Rao M, Das D. Studies on glucoamylase produced from Aspergillus awamori (NRRL-3112) and their effect on saccharification of potato starch. Indian Journal of Experimental Biology. 1995 Dec; 33(12): 957-61en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/58566
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAspergillus --enzymologyen_US
dc.subject.meshGlucan 1,4-alpha-Glucosidase --isolation & purificationen_US
dc.subject.meshSolanum tuberosumen_US
dc.subject.meshStarch --metabolismen_US
dc.titleStudies on glucoamylase produced from Aspergillus awamori (NRRL-3112) and their effect on saccharification of potato starch.en_US
dc.typeJournal Articleen_US
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