Molecular epidemiology of Mycobacterium avium subspecies paratuberculosis in different captive wild animals
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Date
2024-01
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Publisher
Open Science Publishers LLP
Abstract
Molecular epidemiological study of Mycobacterium avium subspecies paratuberculosis from captive wild animals of Siddharth Garden Aurangabad Municipal Corporation Zoo, Maharashtra, was carried out based on individual/pooled fecal samples (n = 42) from different captive wild animal species during winter, summer, and monsoon seasons. Based on microscopy, 29 were positive for acid–fast bacilli out of the total 126 fecal samples screened. Real time-polymerase chain reaction (PCR) targeting IS900 gene detected 14 samples (5 carnivores, 7 herbivores, and 2 omnivores) positive. The overall prevalence of Mycobacterium avium subspecies paratuberculosis (MAP) infection was 11.11% (11.11% carnivores, 29.16% herbivores, 66.66% omnivores, 0% birds, and 0% reptiles). Molecular typing (IS1311 PCR_REA) revealed all positive samples belonged to “Indian Bison type,” biotype. This study first time revealed the presence of MAP infection in tigers from India and highlights the silent interspecies transmission of “Indian Bison type” biotype from herbivores to omnivores and carnivores. The finding observed in this study could help in the control of MAP in captive wild animal species by implementing effective management that will be helpful to minimize spread and reduce burden of this chronic life-threatening disease in captive animal species.
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Keywords
Captive wild animals, Epidemiology, Genotyping, Mycobacterium avium subsp. Paratuberculosis, Polymerase chain reaction REA.
Citation
Lingayat SS, Shafi TA, Naikwade BS., Sakhare MP, Siddiqui MF, Suryawansh PR, Syed Am, Mir AQ, Chaubey KK, Gupta S, Navabharat M, Abdullah M, Rajput P, Singh SV. Molecular epidemiology of Mycobacterium avium subspecies paratuberculosis in different captive wild animals. Journal of Applied Biology & Biotechnology. 2024 Jan; 12(1): 229-233