Simultaneous Estimation Of Mometasone Furoate And Formoterol Fumarate By Hplc Method In Rotacaps

Simple item page
dc.contributor.authorZanwar, A. S.en_US
dc.contributor.authorSen, D. B.en_US
dc.contributor.authorSen, A. K.en_US
dc.contributor.authorSeth, A. K.en_US
dc.date.accessioned2020-09-24T08:00:49Z
dc.date.available2020-09-24T08:00:49Z
dc.date.issued2019-02
dc.description.abstractObjective: To develop and validate a simple and sensitive RP-HPLC method for the simultaneous determination of mometasone furoate (MOM) and formoterol fumarate (FOR) in pharmaceutical dosage forms. Methods: In RP-HPLC method, chromatographic separation was achieved using a mixture of a solvent system consisting of methanol–water (pH 3.5) in the ratio of 85:15 % v/v at a flow rate of 1 ml/min and detection was carried out at 225 nm. Results: The run time for the simultaneous estimation of drugs for the proposed method was 10 min as drugs eluted at 5.217 min (MOM) and 8.650 min (FOR). The linearity was found in the range of 33.33-299.97 μg/ml and 1-9 μg/ml for MOM and FOR, respectively. The values of limit of detection and limit of quantification were 3.634, 0.266 µg/ml and 11.014, 0.807 µg/ml, which indicates the sensitivity of the method for the estimation of MOM and FOR, respectively. The results of recovery studies for both the drugs were within the range i.e. 98.87-101.48 % which indicates the accuracy of the method. Relative standard deviation obtained from repeatability and reproducibility studies were less than 2% indicates the precision of the method. The proposed method was validated according to ICH guidelines. Conclusion: The proposed RP-HPLC method was found to be sensitive and precise because of the low LOD, LOQ and % RSD values (<2). The proposed work does not require acetonitrile and ion pairing reagent as compared to the reported methods. Therefore, method can be used preferably for routine analysis due to its simplicity and economic advantages.en_US
dc.identifier.affiliationsDepartment of Pharmacy, Sumandeep Vidyapeeth University, Piparia, Waghodia, Vadodara 391760, Gujarat, Indiaen_US
dc.identifier.affiliationsDepartment of Pharmacy, Sumandeep Vidyapeeth University, Piparia, Waghodia, Vadodara-391760, Gujarat, Indiaen_US
dc.identifier.affiliationsDepartment of Pharmacy, Sumandeep Vidyapeeth University, Piparia, Waghodia, Vadodara-391760, Gujarat, Indiaen_US
dc.identifier.affiliationsDepartment of Pharmacy, Sumandeep Vidyapeeth University, Piparia, Waghodia, Vadodara-391760, Gujarat, Indiaen_US
dc.identifier.citationZanwar A. S., Sen D. B., Sen A. K., Seth A. K.. Simultaneous Estimation Of Mometasone Furoate And Formoterol Fumarate By Hplc Method In Rotacaps. International Journal of Pharmacy and Pharmaceutical Sciences. 2019 Feb; 11(2): 12-16en_US
dc.identifier.issn0975-1491
dc.identifier.issn2656-0097
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/205825
dc.languageenen_US
dc.publisherInnovare Academic Sciences Pvt. Ltd.en_US
dc.relation.issuenumber2en_US
dc.relation.volume11en_US
dc.source.urihttps://doi.org/10.22159/ijpps.2019v11i2.24799en_US
dc.subjectRP- HPLCen_US
dc.subjectMometasone furoateen_US
dc.subjectFormoterol fumarateen_US
dc.subjectAnalytical methoden_US
dc.titleSimultaneous Estimation Of Mometasone Furoate And Formoterol Fumarate By Hplc Method In Rotacapsen_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijpps2019v11n2p12.pdf
Size:
327.65 KB
Format:
Adobe Portable Document Format
Download
Collections
International Journal of Pharmacy and Pharmaceutical Sciences