The Immunohistochemical Localization Of Bax In The Brain Of Hypothyroid Neonate During Maternal Melatonin Intake

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dc.contributor.authorHidayat, Mariyahen_US
dc.contributor.authorHaider, Ihtishamen_US
dc.contributor.authorKhurram, Abdullahen_US
dc.contributor.authorKhalid, P. Lone.en_US
dc.date.accessioned2020-04-23T07:31:56Z
dc.date.available2020-04-23T07:31:56Z
dc.date.issued2019-07
dc.description.abstractObjective: To immunohistochemically localize Bax proteins in pyramidal cells of cerebral cortex in hypothyroidneonates and to observe the effect of melatonin on these cells.Study Design: Randomized controlled trial.Place and duration of study: Animal House and Anatomy Department at University of Health Sciences, Lahore.Material and Methods: Nine female wistar rats were randomly divided equally into three groups, includingcontrol(A), propylthiouracyl(B), melatonin treated propylthiouracyl (C) group and allowed to conceive. Medicationwas given throughout pregnancy and lactation. After delivery, 10 neonatal rats from each group were sacrificedon 22nd day of life and blood samples were immediately collected from the cardiac region for evaluating serumlevels of TSH. The freshly extracted brains were immediately immersed in neutral buffered formalin for 3 weeksand later further processed. 3 m thick sections were sliced from cerebrum, deparaffinized and rehydrated, thenwashed in phosphate buffered saline and subsequently treated with primary and secondary antibodies.Results: PTU group had significant number of pyramidal neurons in cerebral cortex showing signs of apoptosis,whereas melatonin treated group showed reversal of these signs. Moreover, on serum analysis, the pups of damstaking PTU were severely hypothyroid whereas melatonin treated pups showed significant restoration of serumTSH levels.Conclusion: Melatonin preserves the structure and function of pyramidal neurons of cerebral cortex of neonatesif ingested by hypothyroid mothers during gestation and lactation.en_US
dc.identifier.affiliationsAssociate Professor, Department of Anatomy, Rahbar Medical and Dental College, Lahore, Pakistan.en_US
dc.identifier.affiliationsResearch student at University of Health Sciences, Lahore, Pakistanen_US
dc.identifier.affiliationsTeaching and research fellow, Department of Anatomy, University of Health Sciences, Lahore, Pakistan.en_US
dc.identifier.affiliationsProfessor, Department of Physiology & Cell Biology, University of Health Sciences, Lahore, Pakistan, drmariyah.hidayat@gmail.comen_US
dc.identifier.citationHidayat Mariyah, Haider Ihtisham, Khurram Abdullah, Khalid P. Lone.. The Immunohistochemical Localization Of Bax In The Brain Of Hypothyroid Neonate During Maternal Melatonin Intake. International Journal of Anatomy and Research. 2019 Jul; 3: 6929-6933en_US
dc.identifier.issn2321-4287
dc.identifier.issn2321-8967
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/198619
dc.languageenen_US
dc.publisherIMED Research Publicationsen_US
dc.relation.issuenumber3en_US
dc.relation.volume7en_US
dc.source.urihttps://dx.doi.org/10.16965/ijar.2019.259en_US
dc.subjectMelatoninen_US
dc.subjectPropylthiouracylen_US
dc.subjecthypothyroidismen_US
dc.subjectthyroid stimulating hormoneen_US
dc.subjectanti-bax antibodies.en_US
dc.titleThe Immunohistochemical Localization Of Bax In The Brain Of Hypothyroid Neonate During Maternal Melatonin Intakeen_US
dc.typeJournal Articleen_US
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