Occurrence of non-protein low molecular weight cardiotoxin in Indian King Cobra (Ophiophagus hannah) Cantor 1836, venom.

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dc.contributor.authorSaha, Architaen_US
dc.contributor.authorGomes, Aparnaen_US
dc.contributor.authorGiri, Ben_US
dc.contributor.authorChakravarty, A Ken_US
dc.contributor.authorBiswas, A Ken_US
dc.contributor.authorDasgupta, S Cen_US
dc.contributor.authorGomes, Aen_US
dc.date.accessioned2009-05-28T11:05:57Z
dc.date.available2009-05-28T11:05:57Z
dc.date.issued2006-04-25en_US
dc.description.abstractPathophysiology due to snakebite is a combined effect of various actions of the complex venom constituents. Importance of protein toxins in snake envenomation is well known. The present investigation reports the existence of nonprotein/nonpetide low molecular weight toxin in Indian King Cobra venom, which plays an important role in envenomation consequences in experimental animal models. A group of non-peptidic toxins (OH-NPT1) was isolated from Indian King Cobra Ophiophagus hannah by thin layer chromatography and silica gel column chromatography. UV, IR, NMR and (ESI) TOF-MS studies characterized the OH-NPT1 as a mixture of aliphatic acids having molecular weights 256, 326 and 340Da. The minimum lethal dose of OH-NPT1 was found to be 2.5 microg/20g (iv) and 4microg/20g (ip) in male albino mice. The cardiotoxic property of OH-NPT1 was established through studies on isolated guinea pig heart and auricle preparations, ECG studies in albino rat and estimation of LDH1/LDH and CPK-MB/CPK ratio in Swiss albino mice. Commercial antiserum failed to neutralize the lethality and cardiotoxicity of the toxin. However, calcium and magnesium effectively neutralized the lethal action.en_US
dc.description.affiliationLaboratory of Toxinology and Experimental Pharmacodynamics, Department of Physiology, University of Calcutta, Kolkata, India.en_US
dc.identifier.citationSaha A, Gomes A, Giri B, Chakravarty AK, Biswas AK, Dasgupta SC, Gomes A. Occurrence of non-protein low molecular weight cardiotoxin in Indian King Cobra (Ophiophagus hannah) Cantor 1836, venom. Indian Journal of Experimental Biology. 2006 Apr; 44(4): 279-85en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/56238
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/6419/1/IJEB%2044%284%29%20279-285.pdfen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiological Markersen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshChromatography, Thin Layeren_US
dc.subject.meshCobraen_US
dc.subject.meshCobra Cardiotoxin Proteins --isolation & purificationen_US
dc.subject.meshCobra Venoms --chemistryen_US
dc.subject.meshElectrocardiographyen_US
dc.subject.meshHeart --drug effectsen_US
dc.subject.meshHydrophobicityen_US
dc.subject.meshIndiaen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshMyocardial Contractionen_US
dc.subject.meshProteins --metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshSpectrum Analysisen_US
dc.titleOccurrence of non-protein low molecular weight cardiotoxin in Indian King Cobra (Ophiophagus hannah) Cantor 1836, venom.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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