Differential modification of radiation damage in 5-bromo-2-deoxy-uridine sensitized human glioma cells and PHA-stimulated peripheral leukocytes by 2-deoxy-D-glucose.

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dc.contributor.authorKalia, V Ken_US
dc.contributor.authorDevi, N Ken_US
dc.date.accessioned2009-05-28T13:09:33Z
dc.date.available2009-05-28T13:09:33Z
dc.date.issued1994-09-01en_US
dc.description.abstractEffects of 5-bromo-2-deoxy-uridine (BrdU) and 2-deoxy-D-glucose (2-DG) on 60-Co-gamma-ray induced damage were studied in monolayer cultures of glioma (BMG-1) cells, and PHA-stimulated peripheral leukocytes from normal donors. Micronuclei formation was used as an index of cytogenetic damage. BrdU and 2-DG treatments did not induce micronuclei formation in unirradiated cultures. Presence of BrdU (0.8 microM) for more than one cell cycle (24 hr) significantly increased gamma-ray (1-4 Gy) induced micronuclei formation in exponentially growing BMG-1 cells. Incubation of irradiated cells under sub-optimal growth conditions (DMEM with 1% serum) for 3 hr, instead of growth medium, significantly decreased micronuclei formation. Post-irradiation presence of 2-DG (5 mM; 3 hr, in DMEM + 1% serum) significantly increased radiation damage. In BrdU sensitized cells also, 2-DG significantly increased radiation damage further. In PHA-stimulated leukocytes from normal donors, 2-DG (5mM, equimolar with glucose; for 2 hr) did not increase gamma-ray (2-Gy, 42 hr after PHA-stimulation) induced micronuclei formation. Pre-irradiation presence of BrdU (1.6 microM) significantly increased micronuclei. On the contrary, 2-DG treatment reduced radiation induced micronuclei formation in BrdU sensitized leukocyte cultures. These results suggest that (i) radiation induced lesions leading to micronuclei formation in proliferating tumour cells, are, at least, partly repairable; (ii) combination of 2-DG could reduce BrdU doses required for radiosensitization of proliferating tumour cells; and (iii) 2-DG could differentially increase radiation damage in BrdU sensitized proliferating tumour cells, while reducing manifestation of damage in normal proliferating cells.en_US
dc.description.affiliationDepartment of Biophysics, National Institute of Mental Health and Neurosciences, Bangalore, India.en_US
dc.identifier.citationKalia VK, Devi NK. Differential modification of radiation damage in 5-bromo-2-deoxy-uridine sensitized human glioma cells and PHA-stimulated peripheral leukocytes by 2-deoxy-D-glucose. Indian Journal of Experimental Biology. 1994 Sep; 32(9): 637-42en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/59039
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAdulten_US
dc.subject.meshBromodeoxyuridine --pharmacologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshDeoxyglucose --pharmacologyen_US
dc.subject.meshGlioma --pathologyen_US
dc.subject.meshHumansen_US
dc.subject.meshLeukocytes --drug effectsen_US
dc.subject.meshMaleen_US
dc.subject.meshPhytohemagglutininsen_US
dc.subject.meshRadiation-Sensitizing Agents --pharmacologyen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleDifferential modification of radiation damage in 5-bromo-2-deoxy-uridine sensitized human glioma cells and PHA-stimulated peripheral leukocytes by 2-deoxy-D-glucose.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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