Verotoxic Escherichia coli (STEC) from beef and its products.

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dc.contributor.authorHazarika, R Aen_US
dc.contributor.authorSingh, D Ken_US
dc.contributor.authorKapoor, K Nen_US
dc.contributor.authorAgarwal, R Ken_US
dc.contributor.authorPandey, A Ben_US
dc.contributor.authorPurusottam,en_US
dc.date.accessioned2009-05-28T15:36:44Z
dc.date.available2009-05-28T15:36:44Z
dc.date.issued2007-02-23en_US
dc.description.abstractIn the present investigation, out of 27 (24.10%) strains of Escherichia coli isolated from 112 beef samples comprising raw meat (45), kabab (36) and kofta (31), 9 (33.33%) belonging to 7 different serotypes were verotoxic as tested by vero cell cytotoxic assay. Serotype O145 was the predominant STEC in raw meat. Interestingly, one STEC-O157 strain was also detected. All the STEC strains were positive for Stx genes by polymerase chain reaction showing stx2 (77.78%) to be most predominant followed by stx1 (22.22%). Phenotypic enterohaemolysin production on washed sheep blood agar supplemented with CaCl2 revealed 6 (66.67%) STEC strains to be positive. Presence of STEC in cooked beef products, viz., kabab and kofta appeared to be a matter of concern and potential threat to public health.en_US
dc.description.affiliationDivision of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, India. rah@sancharnet.inen_US
dc.identifier.citationHazarika RA, Singh DK, Kapoor KN, Agarwal RK, Pandey AB, Purusottam . Verotoxic Escherichia coli (STEC) from beef and its products. Indian Journal of Experimental Biology. 2007 Feb; 45(2): 207-11en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/62194
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/5236/1/IJEB%2045%282%29%20207-211.pdfen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCattleen_US
dc.subject.meshCercopithecus aethiopsen_US
dc.subject.meshEscherichia coli --classificationen_US
dc.subject.meshEscherichia coli Proteins --metabolismen_US
dc.subject.meshHemolysin Proteins --metabolismen_US
dc.subject.meshMeat --microbiologyen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshSerotypingen_US
dc.subject.meshShiga Toxin --geneticsen_US
dc.subject.meshVero Cellsen_US
dc.titleVerotoxic Escherichia coli (STEC) from beef and its products.en_US
dc.typeJournal Articleen_US
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