Anti-Inflammatory Effects of Vitex altissima Leaf Extract in Lipopolysaccharide-Induced RAW 264.7 Macrophages

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dc.contributor.authorAzeez, Ajinza A.en_US
dc.contributor.authorRauf, Arun A.en_US
dc.contributor.authorEdwin, Boby T.en_US
dc.date.accessioned2024-09-24T08:59:24Z
dc.date.accessioned2024-09-24T11:51:25Z
dc.date.available2024-09-24T08:59:24Z
dc.date.available2024-09-24T11:51:25Z
dc.date.issued2023-08
dc.description.abstractVitex altissima has been conventionally utilized for its wide-ranging properties in the management of oxidative stress and inflammation. The present investigation was centered on the quantification of the anti-inflammatory efficacy of Vitex altissima. The samples were evaluated for their ability to impede the activity of proteinase, denature proteins, and stabilize the membrane of human red blood cells (HRBC). The present study investigated the inhibitory impact of extracts on the production of total cyclooxygenase, lipoxygenase-5 (5-LOX), myeloperoxidase (MPO), and nitric oxide (NO) using the RAW 264.7 cell line. Furthermore, the antioxidant properties were assessed through the employment of both DPPH assay and reducing power assay. According to the findings, the methanolic extract of Vitex altissima (VAME) was identified as the most efficacious fraction with anti-inflammatory and antioxidant properties. The findings indicate that the extracts exhibited dose-dependent inhibition of proteinase, protein denaturation, and hemolysis of HRBC membrane, which is beneficial. The extracts of Vitex altissima, when treated at concentrations that are not cytotoxic, were observed to have a significant effect in reducing the activity of COX, 5-LOX, and MPO in RAW 264.7 cell line treated with LPS. This resulted in a decrease in NO levels. The dose-dependent increase in in vitro anti-inflammatory activity of Vitex altissima suggests its potential use as a pharmacological agent for the management of diseases related to inflammation. Additional comprehensive phytochemical investigations, in conjunction with in vitro and in vivo analyses, are necessary to identify the active constituent within the extract.en_US
dc.identifier.affiliationsDepartment of Biochemistry, University of Kerala, Kariavattom, Kerala, India; TKM College of Arts and Science, Kollam, Kerala, Indiaen_US
dc.identifier.affiliationsDepartment of Biochemistry, University of Kerala, Kariavattom, Kerala, Indiaen_US
dc.identifier.affiliationsTKM College of Arts and Science, Kollam, Kerala, Indiaen_US
dc.identifier.citationAzeez Ajinza A., Rauf Arun A., Edwin Boby T. . Anti-Inflammatory Effects of Vitex altissima Leaf Extract in Lipopolysaccharide-Induced RAW 264.7 Macrophages. International Journal of Pharmaceutical Sciences and Drug Research . 2023 Jul; 15(4): 461-468en_US
dc.identifier.issn0975–248X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/231703
dc.languageenen_US
dc.publisherMRI Publication Pvt. Ltd.en_US
dc.relation.issuenumber4en_US
dc.relation.volume15en_US
dc.source.urihttps://doi.org/10.25004/IJPSDR.2023.150410en_US
dc.subjectVitex altissimaen_US
dc.subjectantioxidanten_US
dc.subjectinflammationen_US
dc.subjectmacrophagesen_US
dc.subjectLPSen_US
dc.subjectNitriteen_US
dc.titleAnti-Inflammatory Effects of Vitex altissima Leaf Extract in Lipopolysaccharide-Induced RAW 264.7 Macrophagesen_US
dc.typeJournal Articleen_US
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