Abnormal lipid metabolism in collagen-induced arthritis rat model: In vitro, high resolution NMR spectroscopy based analysis.

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dc.contributor.authorSrivastava, Niraj Kumar
dc.contributor.authorSharma, Shikha
dc.contributor.authorPurusottam, Rudra N
dc.contributor.authorSinha, Neeraj
dc.contributor.authorSingh, Rameshwar
dc.contributor.authorSharma, Deepak
dc.date.accessioned2014-12-11T07:36:02Z
dc.date.available2014-12-11T07:36:02Z
dc.date.issued2014-07
dc.description.abstractCollagen-induced arthritis (CIA) was induced in female Wistar rats by intradermal injection of porcine immunization grade native collagen type II (Chondrex). Development and progression of CIA was monitored by studying histopathological, radiographical and biochemical features of arthritic manifestations in the knee joints, hind limb and blood plasma. In addition, oxidative stress status of arthritic animals was determined by measuring lipid peroxidation and the antioxidant enzymes: catalase, superoxide dismutase and glutathione peroxidase. High resolution proton NMR spectroscopy was employed for the analysis of lipid components in the lipid extracts of the joint tissue and plasma of collagen-induced arthritic and control rats. Triglyceride levels showed significant decreases in plasma (1.7 times) but were unchanged in the joint tissue of CIA rats as compared to control. One-dimensional proton NMR spectra showed a 6.2 times reduction in the quantity of choline-containing phospholipids in the plasma of CIA as compared to control rats. There was a 1.6 times elevation of choline-containing phospholipids in the joint tissue of CIA rats as compared to controls. Induction of arthritis showed a 4.0 times reduction in the level of total cholesterol in the plasma and 1.6 times elevation in the joint tissue of CIA rats as compared to controls. The ratio of saturated fatty acids to unsaturated fatty acids was 1.5 times significantly higher in joint tissue and 2.1 times significantly higher in plasma of CIA rats as compared to controls. The results demonstrated significantly altered lipid patterns in the joint tissue and plasma of collagen-induced arthritic rats as detected by one- and two-dimensional NMR spectroscopy compared with controls.en_US
dc.identifier.citationSrivastava Niraj Kumar, Sharma Shikha, Purusottam Rudra N, Sinha Neeraj, Singh Rameshwar, Sharma Deepak. Abnormal lipid metabolism in collagen-induced arthritis rat model: In vitro, high resolution NMR spectroscopy based analysis. Indian Journal of Experimental Biology. 2014 Jul; 52(7): 673-682.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/153747
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/29040en_US
dc.subjectCholesterolen_US
dc.subjectCollagen-induced arthritisen_US
dc.subjectLipid extractionen_US
dc.subjectLipid componentsen_US
dc.subjectMetabolismen_US
dc.subjectNMR spectroscopyen_US
dc.subjectOxidative stressen_US
dc.subjectPhospholipidsen_US
dc.subject.meshAnimals
dc.subject.meshAntioxidants --metabolism
dc.subject.meshArthritis, Experimental --chemically induced
dc.subject.meshArthritis, Experimental --metabolism
dc.subject.meshArthritis, Experimental --pathology
dc.subject.meshBiological Markers --analysis
dc.subject.meshCollagen --toxicity
dc.subject.meshFemale
dc.subject.meshLipid Metabolism
dc.subject.meshLipid Peroxidation
dc.subject.meshMagnetic Resonance Spectroscopy
dc.subject.meshRats
dc.subject.meshRats, Wistar
dc.titleAbnormal lipid metabolism in collagen-induced arthritis rat model: In vitro, high resolution NMR spectroscopy based analysis.en_US
dc.typeArticleen_US
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