Caffeine in tea plants [Camellia sinensis (L) O. Kuntze]: in situ lowering by Bacillus licheniformis (Weigmann) Chester.

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dc.contributor.authorRamarethinam, Sen_US
dc.contributor.authorRajalakshmi, Nen_US
dc.date.accessioned2009-05-28T15:36:02Z
dc.date.available2009-05-28T15:36:02Z
dc.date.issued2004-06-21en_US
dc.description.abstractTea plants (Camellia sinensis) contain 5-6% caffeine that is responsible for the stimulating effect of the beverage. As the tolerance to caffeine varies among individuals, low caffeine tea would be an ideal alternative. While assessing the potential of a few selected bacteria-Bacillus licheniformis, B. subtilis and B. firmus, to multiply on nutrient medium supplemented with glucose (5%) and tea leaf extract (2%), it was observed that only B. licheniformis could proliferate on this medium. Hence, B. licheniformis was used for further studies. Tea plants were sprayed with a suspension of B. licheniformis at a dilution of 5 x 10(8) CFU/ml containing 0.1% Tween 80 as surfactant. In situ lowering of caffeine from tea leaves was evident without affecting the quality of the other tea components. Further, there was no change in the morphological and physiological characteristics as well. It is suggested that spraying of B. licheniformis may be useful in yielding decaffeinated tea with good flavour and aroma.en_US
dc.description.affiliationResearch and Development Centre, United Nilgiri Tea Estates Co Ltd., 3, Savithri Shanmugham Road, Race Course, Coimbatore 641 018, India.en_US
dc.identifier.citationRamarethinam S, Rajalakshmi N. Caffeine in tea plants [Camellia sinensis (L) O. Kuntze]: in situ lowering by Bacillus licheniformis (Weigmann) Chester. Indian Journal of Experimental Biology. 2004 Jun; 42(6): 575-80en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/62180
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshBacillus --metabolismen_US
dc.subject.meshBeveragesen_US
dc.subject.meshCaffeine --pharmacologyen_US
dc.subject.meshCamellia sinensis --metabolismen_US
dc.subject.meshCatechin --metabolismen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshGlucose --metabolismen_US
dc.subject.meshPhenols --chemistryen_US
dc.subject.meshPlant Leavesen_US
dc.subject.meshSpectrophotometryen_US
dc.subject.meshTea --microbiologyen_US
dc.subject.meshTime Factorsen_US
dc.titleCaffeine in tea plants [Camellia sinensis (L) O. Kuntze]: in situ lowering by Bacillus licheniformis (Weigmann) Chester.en_US
dc.typeJournal Articleen_US
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