Natural Alternatives to Chemical Staining in Routine Histopathology - A Narrative Review

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dc.contributor.authorMulla, SAen_US
dc.contributor.authorAnsari, Aen_US
dc.contributor.authorShrivastava, Sen_US
dc.contributor.authorBhattacharjee, Men_US
dc.contributor.authorPanchmahalkar, Aen_US
dc.date.accessioned2024-12-02T11:45:48Z
dc.date.available2024-12-02T11:45:48Z
dc.date.issued2024-01
dc.description.abstractIntroduction: Histopathology is the study of diseases accomplished by fixing tissue onto a glass plate using dyes or stains under a microscope. The commonly used dyes in routine histopathology are Hematoxylin and Eosin (H and E) stain, Gram stain, silver nitrate, Mason’s trichrome stain, Periodic acid–Schiff (PAS) stain, etc. However, one major drawback of these stains are toxicity due to chemicals used. Objectives: The present article reviews the various natural stains that can be used as an alternative to the routinely used stains. Materials and Methods: Data was collected by electronic search of databases including PubMed and Google Scholar for “natural stains”, “natural staining” and “natural stains AND histopathology”. Results: Routinely used stains demonstrate excellent tissue coloring capacity. However, the major drawbacks of routine stains and dyes include use of chemical ingredients, high cost, and some extent of tissue damage. These issues can be resolved by using natural substitutes like turmeric, ginger, henna, beetroot, wood dust etc. Over the past few years, interest in natural options to chemical dyes has grown. Conclusion: This article has highlighted major natural staining alternatives with their advantages and disadvantages along with their efficacy in staining routine histopathological tissue specimensen_US
dc.identifier.affiliationsBharati Vidyapeeth (Deemed to be University) Dental College and Hospital, Sector-7, CBD-Belapur, Navi Mumbai, Maharashtra, India – 400614en_US
dc.identifier.affiliationsBharati Vidyapeeth (Deemed to be University) Dental College and Hospital, Sector-7, CBD-Belapur, Navi Mumbai, Maharashtra, India – 400614en_US
dc.identifier.affiliationsDepartment of Pathology, Bharati Vidyapeeth (Deemed to be University) Dental College and Hospital, Sector-7, CBD-Belapur, Navi Mumbai, Maharashtra, India – 400614en_US
dc.identifier.affiliationsDepartment of Microbiology, Bharati Vidyapeeth (Deemed to be University) Dental College and Hospital, Sector-7, CBD-Belapur, Navi Mumbai, Maharashtra, India – 400614en_US
dc.identifier.affiliationsDepartment of Microbiology, Bharati Vidyapeeth (Deemed to be University) Dental College and Hospital, Sector-7, CBD-Belapur, Navi Mumbai, Maharashtra, India – 400614en_US
dc.identifier.citationMulla SA, Ansari A, Shrivastava S, Bhattacharjee M, Panchmahalkar A. Natural Alternatives to Chemical Staining in Routine Histopathology - A Narrative Review. Oral and Maxillofacial Pathology Journal. 2024 Jan; 15(1): 99-104en_US
dc.identifier.issn0976-1225
dc.identifier.issn2322-0384
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/240526
dc.languageenen_US
dc.publisherKairali Society of Oral & Maxillofacial Pathologistsen_US
dc.relation.issuenumber1en_US
dc.relation.volume15en_US
dc.source.urihttps://ompj.org/files/ompj%2015%201%20_article%2019-aa9eccb1a248733d6d9227929bab481095412381.pdfen_US
dc.subjectHistopathologyen_US
dc.subjectStainingen_US
dc.subjectDyesen_US
dc.subjectH and E Stainen_US
dc.subjectHematoxylinen_US
dc.subjectEosinen_US
dc.subjectTurmericen_US
dc.subjectGingeren_US
dc.subjectNaturalen_US
dc.subjectHistologyen_US
dc.subjectPathology.en_US
dc.titleNatural Alternatives to Chemical Staining in Routine Histopathology - A Narrative Reviewen_US
dc.typeJournal Articleen_US
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