In-vitro Assessment of Cholinesterase Inhibitory and Thrombolytic Activity of Six Available Citrus Fruits in Bangladesh: Relevant for Treating Neurodegenerative Disorder

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dc.contributor.authorBiswas, Kushalen_US
dc.contributor.authorHossain, Saiyaraen_US
dc.contributor.authorAlam, Sanzidaen_US
dc.contributor.authorSultana, Taposhien_US
dc.contributor.authorKhan, Farzanaen_US
dc.contributor.authorDutta, Sanjayen_US
dc.date.accessioned2020-01-02T06:13:40Z
dc.date.available2020-01-02T06:13:40Z
dc.date.issued2018-03
dc.description.abstractAim: Citrus fruits are well known for its medicinal and food value. Aim of this study is to investigate acetylcholinesterase ((AChE)) inhibitory activity, butyrylcholinesterase (BuChE) inhibitory activity, total phenolics, flavonoids, flavonols content and thrombolytic activities of crude methanol extracts of 6 citrus fruits (Citrus limon, Citrus aurantifollia, Citrus bergamia, Citrus maxima, Citrus sinensis and Citrus macroptera). Methods: The fruits were extracted by using methanol as solvent. Ellman’s colourimetric method was applied to determine both cholinesterase inhibitory activities, while folin-ciocalteau reagent (FCR) and aluminium chloride were used to quantify total phenolics, flavonoids, flavonol content of those fruits. Blood clot lysis method was applied for determining the thrombolytic activity of those fruits. Results: All citrus fruits contain a good amount of phenolics, flavonoids and flavonols. C. maxima found more prominent in containing phenolics and flavonols compare to other citrus fruits, with 414.06 ± 2.87 mg Gallic Acid Equivalent/gm and 12.94 ± 1.31 mg Catechin Equivalent/gm dried extract respectively. Citrus sinensis showed the highest content in flavonoids with 21.16± 1.37 mg Catechin 20 Equivalent /gm dried extract. Citrus fruits are also a quality source of cholinesterase inhibitors. All the examined citrus fruits were found capable of inhibiting both acetylcholinesterases (AChE) as well as butyrylcholinesterase (BuChE). C. bergamia was most effective in inhibiting AChE with IC50 of 27.18 µg/ml where C. macroptera was best in inhibiting BuChE (IC50 32.5 µg/ml). But none of the citrus fruits was found fit for thrombolytic activity. Conclusion: Citrus fruits are found the sound in inhibiting AChE and BuChE as well as containing Phenolics, flavonoids and flavonols. But they lack in their thrombolytic activity.en_US
dc.identifier.affiliationsDepartment of Pharmacy, East West University, Bangladeshen_US
dc.identifier.affiliationsDepartment of Pharmacy, East West University, Bangladeshen_US
dc.identifier.affiliationsDepartment of Pharmacy, East West University, Bangladeshen_US
dc.identifier.affiliationsDepartment of Pharmacy, East West University, Bangladeshen_US
dc.identifier.affiliationsDepartment of Pharmacy, East West University, Bangladeshen_US
dc.identifier.affiliationsDepartment of Pharmacy, Khwaja Yunus Ali University, Bangladeshen_US
dc.identifier.citationBiswas Kushal, Hossain Saiyara, Alam Sanzida, Sultana Taposhi, Khan Farzana, Dutta Sanjay. In-vitro Assessment of Cholinesterase Inhibitory and Thrombolytic Activity of Six Available Citrus Fruits in Bangladesh: Relevant for Treating Neurodegenerative Disorder. European Journal of Medicinal Plants. 2018 Mar; 22(3): 1-11en_US
dc.identifier.issn2231-0894
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/189361
dc.languageenen_US
dc.publisherScience Domain Internationalen_US
dc.relation.issuenumber3en_US
dc.relation.volume22en_US
dc.source.urihttps://doi.org/10.9734/EJMP/2018/39693en_US
dc.subjectCitrus fruiten_US
dc.subjectphenolicsen_US
dc.subjectflavonoidsen_US
dc.subjectflavonolsen_US
dc.subjectcholinesterase inhibitionen_US
dc.titleIn-vitro Assessment of Cholinesterase Inhibitory and Thrombolytic Activity of Six Available Citrus Fruits in Bangladesh: Relevant for Treating Neurodegenerative Disorderen_US
dc.typeJournal Articleen_US
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