Hepatoprotective and Nephroprotective Effects of Haronga (Harungana madagascariensis) in Combination with Antioxidant Compounds in Albino Spraque Dawley Rats

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dc.contributor.authorOkwulu, Oluwadunni Joanen_US
dc.contributor.authorAbatan, Matthew Oluwoleen_US
dc.date.accessioned2024-09-24T06:21:58Z
dc.date.available2024-09-24T06:21:58Z
dc.date.issued2023-07
dc.description.abstractAim: The aim of this study was to evaluate the effects of Harungana madagascariensis stem bark ethyl acetate extract with antioxidants on the liver and kidney in rats. Study Design: Experimental. Place and Duration of Study: Department of Veterinary Pharmacology and Toxicology, University of Ibadan (from January-February, 2020). Methodology: This was done using Albino rats (Wistar) grouped into 12 of 5 animals per group for protective studies,ethyl acetate extract (EHM) of the plant at 100mg/kg, 200mg/kg and 500mg/kg in combination with each of Ascorbic acid (200mg/kg), Selenium (1mg/kg) and/or Allopurinol (50mg/kg). Group I-III are control groups, IV-VI EHM/Ascorbic acid, groups VII-IX EHM/Se, and groups X-XII EHM/AL. The combination were administered for 8 days prior acetaminophen (800mg/kg) on day 9. Animals were sacrificed on day 10 and samples were collected for biochemical and histopathological evaluations. Results: The study showed that acetaminophen induced-stress on the liver and kidney causing increase in liver biomarkers such as the aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphate (ALP), creatinine, blood urea, total protein and gamma-glutamyl transferase (GGT). The combination of H. madagasacariensis Ethyl acetate extract with Ascorbic acid, Allopurinol and/or Selenium at different doses causes protective effects by reducing the release of biomarkers and stabilized the elevated enzymes level shown in acetaminophen group. The histopathology of the liver in the acetaminophen group showed moderate portal congestion with mild periportal cellular infiltration and a moderate to severe portal fibrosis, while the kidney showed severe diffuse tubular degeneration and necrosis. Conclusion: The EHM (500 mg/kg and 200 mg/kg) in combination with Ascorbic acid, Allopurinol or Selenium protected the liver and kidney from injury induced by Acetaminophen. The Ethyl acetate extract (EHM) of the plant showed synergy with Ascorbic acid, Selenium and Allopurinol to prevent injury on both organs.en_US
dc.identifier.affiliationsNational Institute for Pharmaceutical Research and Development, Idu, Abuja, Nigeriaen_US
dc.identifier.affiliationsFaculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeriaen_US
dc.identifier.citationOkwulu Oluwadunni Joan, Abatan Matthew Oluwole. Hepatoprotective and Nephroprotective Effects of Haronga (Harungana madagascariensis) in Combination with Antioxidant Compounds in Albino Spraque Dawley Rats . International Journal of Biochemistry Research & Review . 2023 Jul; 32(5): 1-11en_US
dc.identifier.issn2231-086X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/226790
dc.languageenen_US
dc.publisherMs. M. B. Mondalen_US
dc.relation.issuenumber5en_US
dc.relation.volume32en_US
dc.source.urihttps://doi.org/10.9734/ijbcrr/2023/v32i5813en_US
dc.subjectHarungana madagascariensisen_US
dc.subjectbiomarkersen_US
dc.subjectantioxidantsen_US
dc.subjectprotectiveen_US
dc.subjecthistopathologyen_US
dc.titleHepatoprotective and Nephroprotective Effects of Haronga (Harungana madagascariensis) in Combination with Antioxidant Compounds in Albino Spraque Dawley Ratsen_US
dc.typeJournal Articleen_US
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