Flavonoid profile and antioxidant activities of methanolic extract of Hyparrhenia hirta (L.) Stapf.

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dc.contributor.authorBouaziz-Ketata, Hanen
dc.contributor.authorZouari, Nabil
dc.contributor.authorSalah, Hichem Ben
dc.contributor.authorRafrafi, Moez
dc.contributor.authorZeghal, Najiba
dc.date.accessioned2015-06-26T05:52:06Z
dc.date.available2015-06-26T05:52:06Z
dc.date.issued2015-04
dc.description.abstractIn this study, we report isolation of flavonoids, viz., 3-O-methylquercetin, tangeritin, luteolin-7-O-glucoside, luteolin, apigenin-7-O-glucoside, apigenin-8-C-glucoside, luteolin-8-C-glucoside, luteolin-6-C-glucoside, diosmetin and catechin from the methanolic extract of Hyparrhenia hirta employing high performance liquid chromatography and liquid chromatography-electrospray ionization-tandem mass spectrometry. The total phenolic content of H. hirta extract was 105.58 ± 0.1 mg gallic acid equivalents/g of plant extract while the total flavonoid content was 45.20 ± 0.2 mg quercetin equivalents/g of plant extract and the total condensed tannin were 72.35 ± 0.7 mg catechin equivalents/g of plant extract by reference to standard curve. The antioxidant activity was assayed through the antioxidant capacity by phosphomolybdenum assay, the reducing power assay and the radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl method. The extract showed dose dependant activity in all the three assays.en_US
dc.identifier.citationBouaziz-Ketata Hanen, Zouari Nabil, Salah Hichem Ben, Rafrafi Moez, Zeghal Najiba. Flavonoid profile and antioxidant activities of methanolic extract of Hyparrhenia hirta (L.) Stapf. Indian Journal of Experimental Biology. 2015 Apr; 53(4): 208-215.en_US
dc.identifier.issn0975-1009
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/158422
dc.language.isoenen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/31234en_US
dc.subjectApigeninen_US
dc.subjectCatechinen_US
dc.subjectDiosmetinen_US
dc.subjectHPLCen_US
dc.subjectLC-ESI-MSen_US
dc.subjectLuteolinen_US
dc.subjectMethylquercetinen_US
dc.subjectTangeritinen_US
dc.subjectThatching grassen_US
dc.subject.meshAntioxidants --chemistry
dc.subject.meshAntioxidants --pharmacology
dc.subject.meshChromatography, High Pressure Liquid
dc.subject.meshFlavanoids --chemistry
dc.subject.meshFlavanoids --pharmacology
dc.subject.meshMethanol --chemistry
dc.subject.meshPlant Extracts --pharmacology
dc.subject.meshPoaceae --chemistry
dc.subject.meshSpectrometry, Mass, Electrospray Ionization
dc.titleFlavonoid profile and antioxidant activities of methanolic extract of Hyparrhenia hirta (L.) Stapf.en_US
dc.typeArticleen_US
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