Influence of Different Temperature Regimes and Culture Media on the Growth and Sporulation of Alternaria brassicae Causing Alternaria Blight of Mustard
No Thumbnail Available
Date
2023-08
Journal Title
Journal ISSN
Volume Title
Publisher
Ms. M. B. Mondal
Abstract
Mustard (Brassica juncea L.) is the second most important oilseed commodity in India. Alternaria blight (Alternaria brassicae) is one of the devastating fungal pathogen, resulting in severe yield losses to the crop. The present study is conducted in-vitro to know the effect of different temperature regimes and various culture media on the mycelial growth and sporulation of A. brassicae. It is clearly evident from the results that all the tested temperature regimes and culture media showed variation in the colony diameter and other cultural characteristics. The temperature of 25°C significantly encouraged mycelial growth (85.00 mm) and exhibited excellent sporulation of A. brassicae compared to 15 and 35°C. In case of culture media, Potato Dextrose Agar was found to be significantly superior over the other media and registered maximum mycelial growth (82.00 mm) with excellent sporulation. However, Oat Meal Agar supported the minimum mycelial growth (45.00 mm) with fair sporulation. The variation in cultural characteristics (colony color, growth, appearance, and shape etc.) was also observed with various culture media.
Description
Keywords
Temperature, culture media, rapeseed-mustard, A. brassicae, Alternaria blight
Citation
S. Hemalatha, Khan R. U., Sayyeda Sibte, Singh Vaibhav Pratap . Influence of Different Temperature Regimes and Culture Media on the Growth and Sporulation of Alternaria brassicae Causing Alternaria Blight of Mustard. International Journal of Environment and Climate Change. 2023 Aug; 13(8): 1232-1239