Constitutive acetonitrile hydrolysing activity of Nocardia globerula NHB-2: Optimization of production and reaction conditions.

Simple item page
dc.contributor.authorKumar, Hen_US
dc.contributor.authorPrasad, Sen_US
dc.contributor.authorRaj, Jen_US
dc.contributor.authorBhalla, T Cen_US
dc.date.accessioned2009-05-28T12:57:35Z
dc.date.available2009-05-28T12:57:35Z
dc.date.issued2006-03-17en_US
dc.description.abstractNocardia globerula NHB-2 exhibited an intracellular acetonitrile hydrolysing activity (AHA) when cultivated in nutrient broth supplemented with glucose (10.0 g/l) and yeast extract (1.0 g/l), at pH 8.0, 30 degrees C for 21 hr. Maximum AHA was recorded in the culture containing 0.1 M of sodium phosphate buffer, (pH 8.8) at 45 degrees C for 15 min with 600 micromol of acetonitrile and resting cells of N. globerula NHB-2 equivalent to 1.0 ml culture broth. This activity was stable up to 40 degrees C and was completely inactivated at or above 60 degrees C. About five-fold increase in AHA was observed after optimization of culture and reaction conditions. Under the optimized conditions, this organism hydrolyzed various nitriles and amides such as propionitrile, benzonitrile. acetamide, and acrylamide to corresponding acids. This nitrile/amide hydrolysing activity of N. globerula NHB-2 has potential applications in enzymatic synthesis of organic acids and bioremediation of nitriles and amides contaminated soil and water system.en_US
dc.description.affiliationDepartment of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171 005. India.en_US
dc.identifier.citationKumar H, Prasad S, Raj J, Bhalla TC. Constitutive acetonitrile hydrolysing activity of Nocardia globerula NHB-2: Optimization of production and reaction conditions. Indian Journal of Experimental Biology. 2006 Mar; 44(3): 240-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/58777
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/6415/1/IJEB%2044%283%29%20240-245.pdfen_US
dc.subject.meshAcetonitriles --metabolismen_US
dc.subject.meshCatalysisen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshHydrolysisen_US
dc.subject.meshIons --chemistryen_US
dc.subject.meshMetals --chemistryen_US
dc.subject.meshNocardia --drug effectsen_US
dc.subject.meshSubstrate Specificityen_US
dc.subject.meshTemperatureen_US
dc.subject.meshTime Factorsen_US
dc.titleConstitutive acetonitrile hydrolysing activity of Nocardia globerula NHB-2: Optimization of production and reaction conditions.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Indian Journal of Experimental Biology